Biologia de Ceratobasidium spp. associada à doença queima dos fios no chá (Camellia sinensis L.)
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Data
2013-01-22
Autores
Silva, Renato Boreli [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A cultura do chá na região do Vale do Ribeira está localizada ao Sul do Estado de São Paulo, onde se concentra uma grande área de Floresta Mata Atlântica do Brasil. A ‘Queima dos fios’ causada pelo fungo Rhizoctonia binucleada (Ceratobasidium spp.; sin. Pellicularia koleroga) é a principal doença que ataca a cultura. A espécie deste fungo ainda não é totalmente conhecida, bem como as informações sobre sua biologia. Deste modo, o objetivo do presente trabalho foi elucidar aspectos da biologia do fungo, realizando a caracterização citomorfológica, cultural, patogênica e molecular de Rhizoctonia binucleada associada a queima dos fios do chá no Vale do Ribeira - SP. Em uma área de cultivo do chá, rodeado por um fragmento de Floresta Mata Atlântica, realizou-se a coleta de ramos e folhas atacadas pela queima do fio e o isolamento em laboratório, obtendo cinco isolados com características específicas de Rhizoctonia spp. Verificou-se que todos os isolados apresentaram dois núcleos por célula, sendo considerados binucleados. A indução da fase teleomórfica foi realizada, mas não houve sucesso na formação completa das estruturas sexuadas de Ceratobasidium spp., porém, houve escassa formação de himênio e metabasidios por alguns isolados, nos meios de cultura Batata dextrose ágar, Agua ágar, Corn meal ágar e Czapeck dox ágar, sob maior ocorrência na condição de fotoperíodo 12h luz/12h escuro a 24°C. Todos os isolados do chá não apresentaram interação de anastomose de hifas com os isolados padrões de Rhizoctonia binucleada GA-A, GA-Bo, GA-P e GA-Q, não pertencendo a estes grupos. A compatibilidade somática entre os isolados do chá apresentaram a formação de três grupos de compatibilidade somática:...
The tea culture in the Vale do Ribeira is located in south of Sao Paulo State which concentrates the large area of Brazil Atlantic Forest. The ‘Thread blight ' caused by the fungus binucleate Rhizoctonia (Ceratobasidium spp.; Sin. Pellicularia koleroga) is the main disease that attacks the culture. The species of this fungus is not yet fully known, as well as information about its biology. Thus, the aim of this study was to elucidate the fungus biology, by the characterization of cytomorphological, cultural, molecular and pathogenic binucleate Rhizoctonia associated with the tea thread blight in the Ribeira Valley - SP, Brazil. In a area of growing tea, surrounded by a fragment of Atlantic Forest was collect branches and leaves attacked by thread blight and forwarded by laboratory isolation, obtaining five isolates with specific characteristics of Rhizoctonia spp. It was verified that all isolates showed two nuclei per cell being considered binucleate. The teleomorph induction was performed, but there was no success in the complete formation of sexed Ceratobasidium sp structures. However, there was scarce formation of hymenium and metabasidias by some isolates in culture medium Potato dextrose agar, Water agar, Corn meal agar and Czapeck dox agar, under increased occurrence in photoperiod condition 12h light /12h dark at 24 ° C. All isolates of tea showed no interaction of hyphal anastomosis with the standard isolates of binucleate Rhizoctonia GA-A, GA-Bo, GA and GA-P-Q not belonging to these anastomosis groups. The somatic compatibility among isolates of tea showed the formation of three somatic compatibility groups: GCS-1, GCS-2 and GCS-1 among the five isolates showing similarity between the microscopic and macroscopic reactions. The morphological characteristics of the colonies also allowed the... (Complete abstract click electronic access below)
The tea culture in the Vale do Ribeira is located in south of Sao Paulo State which concentrates the large area of Brazil Atlantic Forest. The ‘Thread blight ' caused by the fungus binucleate Rhizoctonia (Ceratobasidium spp.; Sin. Pellicularia koleroga) is the main disease that attacks the culture. The species of this fungus is not yet fully known, as well as information about its biology. Thus, the aim of this study was to elucidate the fungus biology, by the characterization of cytomorphological, cultural, molecular and pathogenic binucleate Rhizoctonia associated with the tea thread blight in the Ribeira Valley - SP, Brazil. In a area of growing tea, surrounded by a fragment of Atlantic Forest was collect branches and leaves attacked by thread blight and forwarded by laboratory isolation, obtaining five isolates with specific characteristics of Rhizoctonia spp. It was verified that all isolates showed two nuclei per cell being considered binucleate. The teleomorph induction was performed, but there was no success in the complete formation of sexed Ceratobasidium sp structures. However, there was scarce formation of hymenium and metabasidias by some isolates in culture medium Potato dextrose agar, Water agar, Corn meal agar and Czapeck dox agar, under increased occurrence in photoperiod condition 12h light /12h dark at 24 ° C. All isolates of tea showed no interaction of hyphal anastomosis with the standard isolates of binucleate Rhizoctonia GA-A, GA-Bo, GA and GA-P-Q not belonging to these anastomosis groups. The somatic compatibility among isolates of tea showed the formation of three somatic compatibility groups: GCS-1, GCS-2 and GCS-1 among the five isolates showing similarity between the microscopic and macroscopic reactions. The morphological characteristics of the colonies also allowed the... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Chá, Fungos - Morfologia, Fungos patogenicos, Fungos na agricultura, Fungi in agriculture
Como citar
SILVA, Renato Boreli. Biologia de Ceratobasidium spp. associada à doença queima dos fios no chá (Camellia sinensis L.). 2013. viii, 64 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agronômicas de Botucatu, 2013.