Efeito de isolados de Fusarium oxysporum não patogênicos na redução da severidade da Murcha-de-Fusário do tomateiro
Carregando...
Data
2003-04
Autores
Silva, Juliano Cesar da [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
A cultura do tomateiro é severamente atacada por diversas doenças, entre elas pode-se destacar a Murcha-de-Fusário. O objetivo do presente trabalho foi o de avaliar a eficiência de isolados de Fusarium oxysporum não patogênicos (Fnp) 233, 233/1, 245, 245/1, 141/3, 251, 251/2, 251/5, 257, originários da Universidade de Torino, Itália, no controle da Murcha-de-Fusário, causada por Fusarium oxysporum f. sp. lycopersici raças 1 e 2 (Fol). Os isolados patogênicos CA4A (Fol - 1), C-21A (Fol - 2), TO11 (Fol - 2) e TO245 (Fol - 2), bem como os isolados não patogênicos, foram cultivados em meio de cultura BD (batata-dextrose) durante 10 dias à 25ºC, com agitação constante de 150rpm. Para se obter estruturas de resistência do fungo 500 ml de inóculo líquido de cada isolado de Fnp foram adicionados em 1000 g de talco e incubados durante 30 dias. Com o intuito de verificar a patogenicidade dos isolados CA4A, C-21A, TO11 e TO245, plântulas de tomateiro cv. Viradoro foram transplantadas para substrato infestado com inóculo líquido nas concentrações de Fol de 103, 104, 105 e 106 conídios mL-1. Após 35 dias do transplante, as mudas foram avaliadas quanto a severidade da doença (Notas variando entre 1 a 6, onde: 1 planta sadia e 6 planta morta), a altura e as massas secas do sistema radicular e da parte aérea das plantas. As concentrações de 106 e 105 conídios mL-1 dos isolados de Fol promoveram a maior severidade da doença, quando comparadas com a testemunha não inoculada. Após determinar a concentração do patógeno, verificou-se a atividade não patogênica dos isolados de Fnp em plantas de tomateiro onde, raízes foram imersas em uma suspensão de conídios dos... .
The tomato is severely is affected by several diseases, among them may be detached the Fusarium wilt. The objective this present research was to evaluate the efficacy of the nonpathogenic Fusarium oxysporum strains (Fnp) 233, 233/1, 245, 245/1, 141/3, 251, 251/2, 251/5, 257, from the University of Torino, Italy, on the control of Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici (Fol) races 1 and 2. The pathogenic strains CA4A race 1, C-21A race 2, TO11 race 2 and TO245 race 2, as well the nonpathogenic strains were growing in liquid media PD (potato-dextrose) during 10 days at 25ºC, with constant agitation at 150 rpm. To obtain resistant structure of the fungi 500 mL of the liquid inoculum with each the Fnp strain were mixed in 1000 g of talc and incubated for 30 days. To evaluate the pathogenic activity of the strains: CA4A (Fol - 1), C-21A (Fol - 2), TO11 (Fol - 2) e TO245 (Fol - 2), tomato seedlings cv. Viradoro susceptible to race 2 of the pathogen were transplanted into the infested substrate with inoculum suspension in the concentrations of Fol 103, 104, 105 e 106 conidia mL-1. After 35 days of the seedlings transplantation were evaluated the disease severity (Notes between 1 and 6, where: 1 health plant e 6 died plant), the height plants and the weight dry material of the root system and aerial part of the plants. The concentrations 106 e 105 conidia mL-1 of the strains de Fol increased the higher severity, when compared with non inoculated plants. After determination the concentration of the pathogen, observed the nonpathogenic activity of the Fnp strains in tomato plants where, roots were dipped in a conidial suspension in the concentration of the 106 conidia mL-1. The plants were growing into pots for the 500 mL capacity of the substrate and after 35 days of the growing in greenhouse were realized the evaluations. The plant... (Complete abstract, click electronic address below).
The tomato is severely is affected by several diseases, among them may be detached the Fusarium wilt. The objective this present research was to evaluate the efficacy of the nonpathogenic Fusarium oxysporum strains (Fnp) 233, 233/1, 245, 245/1, 141/3, 251, 251/2, 251/5, 257, from the University of Torino, Italy, on the control of Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici (Fol) races 1 and 2. The pathogenic strains CA4A race 1, C-21A race 2, TO11 race 2 and TO245 race 2, as well the nonpathogenic strains were growing in liquid media PD (potato-dextrose) during 10 days at 25ºC, with constant agitation at 150 rpm. To obtain resistant structure of the fungi 500 mL of the liquid inoculum with each the Fnp strain were mixed in 1000 g of talc and incubated for 30 days. To evaluate the pathogenic activity of the strains: CA4A (Fol - 1), C-21A (Fol - 2), TO11 (Fol - 2) e TO245 (Fol - 2), tomato seedlings cv. Viradoro susceptible to race 2 of the pathogen were transplanted into the infested substrate with inoculum suspension in the concentrations of Fol 103, 104, 105 e 106 conidia mL-1. After 35 days of the seedlings transplantation were evaluated the disease severity (Notes between 1 and 6, where: 1 health plant e 6 died plant), the height plants and the weight dry material of the root system and aerial part of the plants. The concentrations 106 e 105 conidia mL-1 of the strains de Fol increased the higher severity, when compared with non inoculated plants. After determination the concentration of the pathogen, observed the nonpathogenic activity of the Fnp strains in tomato plants where, roots were dipped in a conidial suspension in the concentration of the 106 conidia mL-1. The plants were growing into pots for the 500 mL capacity of the substrate and after 35 days of the growing in greenhouse were realized the evaluations. The plant... (Complete abstract, click electronic address below).
Descrição
Palavras-chave
Tomate - Cultivo, Fusarium oxysporum, Fungos - Controle biológico
Como citar
SILVA, Juliano Cesar da. Efeito de isolados de Fusarium oxysporum não patogênicos na redução da severidade da Murcha-de-Fusário do tomateiro. 2003. xvi, 55 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agronômicas, 2003.