Utilização de processos oxidativos avançados baseados em ozônio como pré-tratamento para bagaço de cana-de-açúcar, e hidrólise do bagaço pré-tratado com combinado enzimático dos fungos Penicillium viridicatum RFC3 e Trichoderma reesei QM9414
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2011-09-14
Autores
Travaini, Rodolfo [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Neste trabalho foi estudada a utilização do ozônio como pré-tratamento do bagaço de cana-de-açúcar para posterior hidrólise enzimática. O bagaço in natura foi caracterizado quimicamente, e após cada ensaio de pré-tratamento foram quantificados no bagaço pré-tratado: celulose, xilana, lignina ácida insolúvel (LAI), lignina ácida sóluvel (LAS) e lignina total (LT). Após os pré-tratamentos as amostras foram hidrolisadas enzimaticamente por mistura dos complexos NS50013 e NS50010 de enzimas comerciais fornecidas pela Novozymes. Foram realizados quatro ensaios de pré-tratamento, variando-se a umidade e a concentração de ozônio (gO3/h), e mantendo-se constante o fluxo 60 L/h e o tamanho de partícula em 3-5 mm. A melhor condição de ensaios encontrada foi com 40 % de umidade e 3,85 gO3/h, onde houve diminuição na quantidade de LAI de 19,54±0,03 para 6,49±0,03 %, aumento na LAS de 3,13±0,04 para 7,21±0,09 % e diminuição da LT de 22,67±0,07 para 13,70±0,12 %, comparando-se com o bagaço in natura. No mesmo ensaio houve uma conversão de hidrólise enzimática da celulose de 39,27±0,32 % no bagaço pré-tratado e 50,62±1,59 % no pré-tratado e lavado, da xilana de 55,90±0,14 % no pré-tratado e 28,14±0,75 % no pré-tratado lavado, de açúcares de 44,61±0,17 % no pré-tratado e 43,41±0,84 % no pré-tratado lavado. Houve pouco ataque do ozônio aos carboidratos em todos os ensaios, sendo o maior ataque no ensaio de melhores rendimentos supracitado, com perda de menos de 5 pontos percentuais na quantidade de celulose e pouco mais de 1 ponto percentual na quantidade de xilana. A análise de inibidores revelou baixas concentrações destes, sendo o de maior concentração o ácido acético, com 1,40040±0,07359 g·L-1 no hidrolisado. A análise das amostras pré-tratadas por micrografia eletrônica de varredura...
In this work was studied the utilization of the ozone as a pretreatment of the sugarcane bagasse for enzymatic hydrolysis. The in natura bagasse was chemically characterized, and after each pretreatment test was quantified in the pretreated bagasse: cellulose, xylan, acid insoluble lignin (AIL), acid soluble lignin (ASL) and total lignin (TL). After the pretreatments the samples was enzymatic hydrolyzed by a mixture of NS50013 and NS50010 complexes, of commercial enzymes provided by Novozymes. Was realize four tests of pretreatment, changing the moisture and ozone concentration (gO3/h), fixing the flux in 60 L/h and the particle size at 3-5 mm. The best condition of test was find at 40 % of moisture and 3,85 gO3/h, where was a diminution in the AIL from 19,54±0,03 to 6,49±0,03, a increase in the ASL from 3,13±0,04 to 7,21±0,09 %, and diminution of LT from 22,67±0,07 to 13,70±0,12 %, comparing to the in natura bagasse. In the same test was a conversion of enzymatic hydrolysis of the cellulose of 39,27±0,32 % in the pretreated bagasse and 50,62±1,59 % in the pretreated and washed, of xilan 55,90±0,14 % in the pretreated bagasse and 28,14±0,75 % in the pretreated and washed, of sugar 44,61±0,17 % in the pretreated bagasse and 43,41±0,84 % in the pretreated and washed. Was a little attack to the carbohydrates in all the tests, being the major attack in the test of the best yields aforementioned, with loss of less than 5 percentage points in the cellulose and little more than 1 percentage point in the amount of xylan. The inhibitors analysis showed slow concentrations of they, being the major concentration of acetic acid, with 1,40040±0,07359 g·L-1 in the hydrolyzed. The electron micrograph analysis of the pretreated samples showed that the ozone pretreatment is able to modify microscopically the bagasse, exposing the fibers and increasing the amount and size of the pores
In this work was studied the utilization of the ozone as a pretreatment of the sugarcane bagasse for enzymatic hydrolysis. The in natura bagasse was chemically characterized, and after each pretreatment test was quantified in the pretreated bagasse: cellulose, xylan, acid insoluble lignin (AIL), acid soluble lignin (ASL) and total lignin (TL). After the pretreatments the samples was enzymatic hydrolyzed by a mixture of NS50013 and NS50010 complexes, of commercial enzymes provided by Novozymes. Was realize four tests of pretreatment, changing the moisture and ozone concentration (gO3/h), fixing the flux in 60 L/h and the particle size at 3-5 mm. The best condition of test was find at 40 % of moisture and 3,85 gO3/h, where was a diminution in the AIL from 19,54±0,03 to 6,49±0,03, a increase in the ASL from 3,13±0,04 to 7,21±0,09 %, and diminution of LT from 22,67±0,07 to 13,70±0,12 %, comparing to the in natura bagasse. In the same test was a conversion of enzymatic hydrolysis of the cellulose of 39,27±0,32 % in the pretreated bagasse and 50,62±1,59 % in the pretreated and washed, of xilan 55,90±0,14 % in the pretreated bagasse and 28,14±0,75 % in the pretreated and washed, of sugar 44,61±0,17 % in the pretreated bagasse and 43,41±0,84 % in the pretreated and washed. Was a little attack to the carbohydrates in all the tests, being the major attack in the test of the best yields aforementioned, with loss of less than 5 percentage points in the cellulose and little more than 1 percentage point in the amount of xylan. The inhibitors analysis showed slow concentrations of they, being the major concentration of acetic acid, with 1,40040±0,07359 g·L-1 in the hydrolyzed. The electron micrograph analysis of the pretreated samples showed that the ozone pretreatment is able to modify microscopically the bagasse, exposing the fibers and increasing the amount and size of the pores
Descrição
Palavras-chave
Microbiologia, Enzimas de fungos, Bagaço de cana, Hidrólise enzimática, Sugarcane bagasse, Enzymatic hydrolysis
Como citar
TRAVAINI, Rodolfo. Utilização de processos oxidativos avançados baseados em ozônio como pré-tratamento para bagaço de cana-de-açúcar, e hidrólise do bagaço pré-tratado com combinado enzimático dos fungos Penicillium viridicatum RFC3 e Trichoderma reesei QM9414. 2011. 62 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências, Letras e Ciências Exatas, 2011.