Development and characterization of 14 microsatellite loci from an enriched genomic library of Eucalyptus camaldulensis Dehnh

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dc.contributor.authorda Silva, Janete Motta [UNESP]
dc.contributor.authorBarbosa de Sousa, Adna Cristina
dc.contributor.authorde Souza, Anete Pereira
dc.contributor.authorMori, Edson Seizo [UNESP]
dc.contributor.authorMenezes Freitas, Miguel Luiz
dc.contributor.authorSebbenn, Alexandre Magno
dc.contributor.authorTeixeira de Moraes, Mario Luiz [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionInst Florestal São Paulo
dc.date.accessioned2014-05-20T13:30:42Z
dc.date.available2014-05-20T13:30:42Z
dc.date.issued2009-12-01
dc.description.abstractEucalyptus camaldulensis Dehnh is an Australian tree species which occurs in various climatic and environmental conditions and show large genetic diversity. Twenty five microsatellite markers were developed from a CT(8)-GT(8) enriched genomic library of E. camaldulensis. The number of alleles ranged from 4 to 13 (average of 8). The polymorphism information content (PIC) and the discriminating power (D) of each primer ranged from 0.37 to 0.88 (average of 0.72) and 0.48 to 0.99 (average of 0.84), respectively. The observed and expected heterozygosities ranged from 0.28 to 0.84 and 0.25 to 0.90, respectively. Four loci showed statistically significant from Hardy-Weinberg equilibrium after Bonferroni correction (P, (5%) < 0.0038). All polymorphic markers were tested for cross-amplification in 25 different Eucalyptus species. Those microsatellite loci will be useful to investigate questions of genetic diversity and structure, gene flow, mating system and ex situ genetic conservation of E. camaldulensis.en
dc.description.affiliationUniv Estadual Paulista UNESP, Dept Fitotecnia Tecnol Alimentos & Socioecon, Fac Engn Ilha Solteira, BR-15385000 Ilha Solteira, SP, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Fac Engn, BR-15385000 Ilha Solteira, SP, Brazil
dc.description.affiliationUniv Estadual Campinas Unicamp, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas Unicamp, Dept Biol Vegetal, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Fac Ciencias Agron, BR-18610307 Botucatu, SP, Brazil
dc.description.affiliationInst Florestal São Paulo, BR-01059970 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Dept Fitotecnia Tecnol Alimentos & Socioecon, Fac Engn Ilha Solteira, BR-15385000 Ilha Solteira, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Fac Engn, BR-15385000 Ilha Solteira, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Fac Ciencias Agron, BR-18610307 Botucatu, SP, Brazil
dc.description.sponsorshipSão Paulo State Government
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 05/58721-9
dc.description.sponsorshipIdFAPESP: 05/59217-2
dc.format.extent465-469
dc.identifierhttp://dx.doi.org/10.1007/s12686-009-9107-7
dc.identifier.citationConservation Genetics Resources. Dordrecht: Springer, v. 1, n. 1, p. 465-469, 2009.
dc.identifier.doi10.1007/s12686-009-9107-7
dc.identifier.issn1877-7252
dc.identifier.lattes9803426672221802
dc.identifier.urihttp://hdl.handle.net/11449/10428
dc.identifier.wosWOS:000207922400105
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofConservation Genetics Resources
dc.relation.ispartofjcr0.742
dc.relation.ispartofsjr0,430
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectConservation geneticen
dc.subjectHardwoodsen
dc.subjectMolecular markeren
dc.subjectMicrosatellitesen
dc.subjectCross-species amplificationen
dc.titleDevelopment and characterization of 14 microsatellite loci from an enriched genomic library of Eucalyptus camaldulensis Dehnhen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes9803426672221802
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Engenharia, Ilha Solteirapt

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Artigos - Fitotecnia, Tecnologia de Alimentos e Sócio Economia - FEIS