The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis

Página do item simplificado
dc.contributor.authorMimura, Kallyne K.
dc.contributor.authorTedesco, Roberto C.
dc.contributor.authorCalabrese, Katia S.
dc.contributor.authorGil, Cristiane D.
dc.contributor.authorOliani, Sonia M. [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInstituto Oswaldo Cruz/FIOCRUZ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:26:51Z
dc.date.available2014-05-27T11:26:51Z
dc.date.issued2012-06-15
dc.description.abstractPurpose: The aim of this study was to evaluate the expression of the protein annexin A1 (ANXA1), a potent endogenous regulator of the inflammatory process, in ocular toxoplasmosis. Methods: C57BL/6 female mice were infected using intravitreal injections of either 10 6 tachyzoites of Toxoplasma gondii (RH strain; T. gondii) or PBS only (control groups). After 24, 48, and 72 h, animals were sacrificed and their eyes were harvested for histopathological, immunohistochemical, and ultrastructural immunocytochemical analysis of ANXA1. Human retinal pigment epithelial (RPE) cells (ARPE-19) were infected in vitro with T. gondii and collected after 60, 120, 240 min, and 24 h. Results: Compared with non-infected eyes, an intense inflammatory response was observed in the anterior (24 h after infection) and posterior segments (72 h after infection) of the infected eye, characterized by neutrophil infiltration and by the presence of tachyzoites and their consequent destruction along with disorganization of normal retina architecture and RPE vacuolization. T. gondii infection was associated with a significant increase of ANXA1 expression in the neutrophils at 24, 48, and 72 h, and in the RPE at 48 and 72 h. In vitro studies confirmed an upregulation of ANXA1 levels in RPE cells, after 60 and 120 min of infection with T. gondii. Conclusions: The positive modulation of endogenous ANXA1 in the inflammatory and RPE cells during T. gondii infection suggests that this protein may serve as a therapeutic target in ocular toxoplasmosis. © 2012 Molecular Vision.en
dc.description.affiliationFrom the Post-Graduation Structural and Functional Biology Federal University of São Paulo (UNIFESP), São Paulo, SP
dc.description.affiliationDepartment of Morphology and Genetics Federal University of São Paulo (UNIFESP), São Paulo, SP
dc.description.affiliationLaboratório de Imunomodulação e Protozoologia Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, RJ
dc.description.affiliationImmunomorphology Laboratory, Department of Biology Instituto de Biociências Letras e Ciências Exatas (IBILCE), São Paulo State University (UNESP), São José do Rio Preto, SP
dc.description.affiliationUnespImmunomorphology Laboratory, Department of Biology Instituto de Biociências Letras e Ciências Exatas (IBILCE), São Paulo State University (UNESP), São José do Rio Preto, SP
dc.format.extent1583-1593
dc.identifierhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3382303/
dc.identifier.citationMolecular Vision, v. 18, p. 1583-1593.
dc.identifier.file2-s2.0-84863323791.pdf
dc.identifier.issn1090-0535
dc.identifier.scopus2-s2.0-84863323791
dc.identifier.urihttp://hdl.handle.net/11449/73384
dc.language.isoeng
dc.relation.ispartofMolecular Vision
dc.relation.ispartofjcr2.219
dc.relation.ispartofsjr1,036
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectlipocortin 1
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectcell vacuole
dc.subjectcontrolled study
dc.subjectdisease association
dc.subjecteye inflammation
dc.subjectfemale
dc.subjectin vitro study
dc.subjectmouse
dc.subjectneutrophil chemotaxis
dc.subjectnonhuman
dc.subjectpigment epithelium
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectretina
dc.subjecttachyzoite
dc.subjectToxoplasma gondii
dc.subjecttoxoplasmosis
dc.subjectupregulation
dc.subjectAnimals
dc.subjectAnnexin A1
dc.subjectAnterior Eye Segment
dc.subjectEpithelial Cells
dc.subjectFemale
dc.subjectGene Expression Regulation
dc.subjectHumans
dc.subjectInterferon-gamma
dc.subjectIntravitreal Injections
dc.subjectMice
dc.subjectMice, Inbred C57BL
dc.subjectNeutrophil Infiltration
dc.subjectPosterior Eye Segment
dc.subjectRetinal Pigment Epithelium
dc.subjectToxoplasma
dc.subjectToxoplasmosis, Animal
dc.subjectToxoplasmosis, Ocular
dc.titleThe involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosisen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
2-s2.0-84863323791.pdf
Tamanho:
6.44 MB
Formato:
Adobe Portable Document Format
Baixar

Coleções

Artigos - Biologia - IBILCE