Light microscopic evaluation and scanning electron microscopic analysis of horse eyes following deep anterior lamellar keratectomy

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dc.contributor.authorMartins, Bianca C. [UNESP]
dc.contributor.authorBrooks, Dennis E.
dc.contributor.authorPlummer, Caryn E.
dc.contributor.authorSamuelson, Don A.
dc.contributor.authorMangan, Brendan G.
dc.contributor.authorLaus, José L. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Florida
dc.date.accessioned2014-05-27T11:29:50Z
dc.date.available2014-05-27T11:29:50Z
dc.date.issued2013-07-01
dc.description.abstractObjective To describe the technique of deep anterior lamellar keratoplasty (DALK) with Descemet's membrane (DM) exposure in horse eyes. Also, to compare the efficacy and safety of viscodissection and big-bubble techniques for DALK. Animals studied Thirty-four ex vivo horse eyes. Procedure Deep anterior lamellar keratoplasty was performed in 34 ex vivo horse eyes. Two groups (Group V - viscodissection - 2% sodium hyaluronate; Group A - air - big-bubble) of 17 eyes were studied. Other than the substance used, the surgical technique was similar for both groups. Nonperforated eyes were submitted for light microscopic histologic evaluation and scanning electron microscopic (SEM) analysis. Results Group V - Perforations occurred in 18% of the eyes during surgery. Light microscopy revealed exposure of DM in 28% of the eyes with mean thickness of the remaining stroma being 70.4μm. Group A - Perforations occurred in 42% of the eyes. Light microscopy revealed exposure of DM in 60% of the eyes with mean thickness of the remaining stroma being 23.3μm. No significant differences in safety, efficacy and thickness of the remaining stroma (including all eyes or excluding those with DM exposure) were observed. SEM of the surgical site revealed a more even surface in those eyes with DM exposure compared to eyes with thicker remaining stroma in both groups. Conclusions We describe two DALK techniques (viscodissection and big-bubble) for use in horses. No significant differences in safety, efficacy and thickness of the remaining stroma were observed. However, a nonsignificant trend toward the big-bubble technique being more efficacious but less safe was observed. © 2012 American College of Veterinary Ophthalmologists.en
dc.description.affiliationCollege of Agricultural and Veterinarian Sciences (FCAV) Sao Paulo State University (UNESP), Jaboticabal, SP
dc.description.affiliationDepartments of Small and Large Animal Clinical Sciences College of Veterinary Medicine University of Florida, Gainesville, FL
dc.description.affiliationUnespCollege of Agricultural and Veterinarian Sciences (FCAV) Sao Paulo State University (UNESP), Jaboticabal, SP
dc.format.extent42-51
dc.identifierhttp://dx.doi.org/10.1111/j.1463-5224.2012.01071.x
dc.identifier.citationVeterinary Ophthalmology, v. 16, n. SUPPL.1, p. 42-51, 2013.
dc.identifier.doi10.1111/j.1463-5224.2012.01071.x
dc.identifier.issn1463-5216
dc.identifier.issn1463-5224
dc.identifier.scopus2-s2.0-84880109937
dc.identifier.urihttp://hdl.handle.net/11449/75811
dc.identifier.wosWOS:000321329000005
dc.language.isoeng
dc.relation.ispartofVeterinary Ophthalmology
dc.relation.ispartofjcr1.083
dc.relation.ispartofsjr0,692
dc.relation.ispartofsjr0,692
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCorneal transplant
dc.subjectDeep anterior lamellar keratoplasty
dc.subjectHistology
dc.subjectHorse
dc.subjectKeratoplasty
dc.subjectScanning electron microscopic
dc.titleLight microscopic evaluation and scanning electron microscopic analysis of horse eyes following deep anterior lamellar keratectomyen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.author.orcid0000-0003-1932-8818[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Clínica e Cirurgia Veterinária - FCAV