First description of the karyotype and localization of major and minor ribosomal genes in Rhoadsia altipinna Fowler, 1911 (Characiformes, Characidae) from Ecuador

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dc.contributor.authorSanchez-Romero, Omar
dc.contributor.authorAbad, Cesar Quezada
dc.contributor.authorCordero, Patricio Quizhpe
dc.contributor.authorSene, Viviani Franca de [UNESP]
dc.contributor.authorNirchio, Mauro
dc.contributor.authorOliveira, Claudio [UNESP]
dc.contributor.institutionUniversidad Técnica de Machala
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidad de Oriente
dc.date.accessioned2015-10-21T13:11:38Z
dc.date.available2015-10-21T13:11:38Z
dc.date.issued2015-01-01
dc.description.abstractKaryotypic features of Rhoadsia altipinna Fowler, 1911 from Ecuador were investigated by examining metaphase chromosomes through Giemsa staining, C-banding, Ag-NOR, and two-color-fluorescence in situ hybridization (FISH) for mapping of 18S and 5S ribosomal genes. The species exhibit a karyotype with 2n = 50, composed of 10 metacentric, 26 submetacentric and 14 subtelocentric elements, with a fundamental number FN=86 and is characterized by the presence of a larger metacentric pair (number 1), which is about 2/3 longer than the average length of the rest of the metacentric series. Sex chromosomes were not observed. Heterochromatin is identifiable on 44 chromosomes, distributed in paracentromeric position near the centromere. The first metacentric pair presents two well-defined heterochromatic blocks in paracentromeric position, near the centromere. Impregnation with silver nitrate showed a single pair of Ag-positive NORs localized at terminal regions of the short arms of the subtelocentric chromosome pair number 12. FISH assay confirmed these localization of NORs and revealed that minor rDNA clusters occur interstitially on the larger metacentric pair number 1. Comparison of results here reported with those available on other Characidae permit to hypothesize that the presence of a very large metacentric pair might represent a unique and derived condition that characterize one of four major lineages molecularly identified in this family.en
dc.description.affiliationUniv Tecn Machala, El Oro, Ecuador
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Morfol, Lab Biol &Genet Peixes, BR-18618970 Sao Paulo, Brazil
dc.description.affiliationUniv Oriente, Escuela Ciencias Aplicadas Mar, Estado Nueva Esparta, Venezuela
dc.description.affiliationUNMSM, Lima, Peru
dc.description.affiliationUnespUniversidade Estadual Paulista, Inst Biociencias Botucatu, Dept Morfologia, Lab Biol &Genet Peixes, BR-18618970 Sao Paulo, Brazil
dc.description.sponsorshipSecretaria de Educacion Superior, Ciencia, Tecnologia e Innovacion of Republica de Ecuador
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent271-280
dc.identifierhttp://compcytogen.pensoft.net/articles.php?id=4504
dc.identifier.citationComparative Cytogenetics. Sofia: Pensoft Publ, v. 9, n. 2, p. 271-280, 2015.
dc.identifier.doi10.3897/CompCytogen.v9i2.4504
dc.identifier.issn1993-0771
dc.identifier.urihttp://hdl.handle.net/11449/128625
dc.identifier.wosWOS:000356049800008
dc.language.isoeng
dc.publisherPensoft Publ
dc.relation.ispartofComparative Cytogenetics
dc.relation.ispartofjcr1.319
dc.relation.ispartofsjr0,431
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subject18S and 5S ribosomal genesen
dc.subjectC-bandsen
dc.subjectfishesen
dc.subjectKaryotypeen
dc.subjectNORsen
dc.titleFirst description of the karyotype and localization of major and minor ribosomal genes in Rhoadsia altipinna Fowler, 1911 (Characiformes, Characidae) from Ecuadoren
dc.typeArtigo
dcterms.rightsHolderPensoft Publ
unesp.author.orcid0000-0001-7171-2433[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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