A hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells

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dc.contributor.authorCavalcanti, Bruno N. [UNESP]
dc.contributor.authorZeitlin, Benjamin D.
dc.contributor.authorNör, Jacques E.
dc.contributor.institutionUniversity of Michigan
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of the Pacific
dc.date.accessioned2014-05-27T11:27:28Z
dc.date.available2014-05-27T11:27:28Z
dc.date.issued2013-01-01
dc.description.abstractObjectives: The clinical translation of stem cell-based Regenerative Endodontics demands further development of suitable injectable scaffolds. Puramatrix™ is a defined, self-assembling peptide hydrogel which instantaneously polymerizes under normal physiological conditions. Here, we assessed the compatibility of Puramatrix™ with dental pulp stem cell (DPSC) growth and differentiation. Methods: DPSC cells were grown in 0.05-0.25% Puramatrix™. Cell viability was measured colorimetrically using the WST-1 assay. Cell morphology was observed in 3D modeling using confocal microscopy. In addition, we used the human tooth slice model with Puramatrix™ to verify DPSC differentiation into odontoblast-like cells, as measured by expression of DSPP and DMP-1. Results: DPSC survived and proliferated in Puramatrix™ for at least three weeks in culture. Confocal microscopy revealed that cells seeded in Puramatrix™ presented morphological features of healthy cells, and some cells exhibited cytoplasmic elongations. Notably, after 21 days in tooth slices containing Puramatrix™, DPSC cells expressed DMP-1 and DSPP, putative markers of odontoblastic differentiation. Significance: Collectively, these data suggest that self-assembling peptide hydrogels might be useful injectable scaffolds for stem cell-based Regenerative Endodontics. © 2012 Academy of Dental Materials.en
dc.description.affiliationDepartment of Cariology, Restorative Sciences, Endodontics University of Michigan School of Dentistry, 1011 N University, Ann Arbor, MI 48109-1078
dc.description.affiliationDepartment of Restorative Dentistry São Paulo State University, São José Dos Campos
dc.description.affiliationArthur A. Dugoni School of Dentistry University of the Pacific, San Francisco, CA
dc.description.affiliationDepartment of Biomedical Engineering University of Michigan College of Engineering
dc.description.affiliationDepartment of Otolaryngology University of Michigan School of Medicine
dc.description.affiliationUnespDepartment of Restorative Dentistry São Paulo State University, São José Dos Campos
dc.format.extent97-102
dc.identifierhttp://dx.doi.org/10.1016/j.dental.2012.08.002
dc.identifier.citationDental Materials, v. 29, n. 1, p. 97-102, 2013.
dc.identifier.doi10.1016/j.dental.2012.08.002
dc.identifier.issn0109-5641
dc.identifier.scopus2-s2.0-84871014475
dc.identifier.urihttp://hdl.handle.net/11449/74169
dc.identifier.wosWOS:000312269000008
dc.language.isoeng
dc.relation.ispartofDental Materials
dc.relation.ispartofjcr4.039
dc.relation.ispartofsjr2,106
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectDental pulp
dc.subjectHydrogel
dc.subjectOdontoblast
dc.subjectRegenerative Endodontics
dc.subjectStem cells
dc.subjectTissue engineering
dc.subject3-d modeling
dc.subjectCell morphology
dc.subjectCell viability
dc.subjectCell-based
dc.subjectClinical translation
dc.subjectHuman teeths
dc.subjectHydrogel scaffolds
dc.subjectInjectable scaffolds
dc.subjectMorphological features
dc.subjectPhysiological condition
dc.subjectPuraMatrix
dc.subjectSelf-assembling peptides
dc.subjectCell culture
dc.subjectConfocal microscopy
dc.subjectDentistry
dc.subjectHydrogels
dc.subjectScaffolds (biology)
dc.titleA hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cellsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

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Artigos - Odontologia Restauradora - ICT