Subcutaneous tissue reaction and cytotoxicity of polyvinylidene fluoride and polyvinylidene fluoride-trifluoroethylene blends associated with natural polymers

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dc.contributor.authorMarques, Leonardo
dc.contributor.authorHolgado, Leandro A.
dc.contributor.authorSimões, Rebeca D. [UNESP]
dc.contributor.authorPereira, João D. A. S. [UNESP]
dc.contributor.authorFloriano, Juliana F. [UNESP]
dc.contributor.authorMota, Lígia S. L. S. [UNESP]
dc.contributor.authorGraeff, Carlos Frederico de Oliveira [UNESP]
dc.contributor.authorConstantino, Carlos J. L. [UNESP]
dc.contributor.authorRodriguez-Perez, Miguel. A.
dc.contributor.authorMatsumoto, Mariza
dc.contributor.authorKinoshita, Angela
dc.contributor.institutionUniversidade do Sagrado Coração (USC)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Valladolid
dc.date.accessioned2014-05-27T11:30:45Z
dc.date.available2014-05-27T11:30:45Z
dc.date.issued2013-10-01
dc.description.abstractCytotoxicity and subcutaneous tissue reaction of innovative blends composed by polyvinylidene fluoride and polyvinylidene fluoride-trifluoroethylene associated with natural polymers (natural rubber and native starch) forming membranes were evaluated, aiming its applications associated with bone regeneration. Cytotoxicity was evaluated in mouse fibroblasts culture cells (NIH3T3) using trypan blue staining. Tissue response was in vivo evaluated by subcutaneous implantation of materials in rats, taking into account the presence of necrosis and connective tissue capsule around implanted materials after 7, 14, 21, 28, 35, 60, and 100 days of surgery. The pattern of inflammation was evaluated by histomorphometry of the inflammatory cells. Chemical and morphological changes of implanted materials after 60 and 100 days were evaluated by Fourier transform infrared (FTIR) absorption spectroscopy and scanning electron microscopy (SEM) images. Cytotoxicity tests indicated a good tolerance of the cells to the biomaterial. The in vivo tissue response of all studied materials showed normal inflammatory pattern, characterized by a reduction of polymorphonuclear leukocytes and an increase in mononuclear leukocytes over the time (p < 0.05 Kruskal-Wallis). On day 60, microscopic analysis showed regression of the chronic inflammatory process around all materials. FTIR showed no changes in chemical composition of materials due to implantation, whereas SEM demonstrated the delivery of starch in the medium. Therefore, the results of the tests performed in vitro and in vivo show that the innovative blends can further be used as biomaterials. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 1284-1293, 2013. Copyright © 2013 Wiley Periodicals, Inc.en
dc.description.affiliationUniversidade Sagrado Coração - USC, São Paulo, Bauru
dc.description.affiliationFaculdade de Ciências e Tecnologia UNESP Universidade Estadual Paulista, São Paulo, Presidente Prudente
dc.description.affiliationFaculdade de Ciências UNESP Universidade Estadual Paulista, São Paulo, Bauru
dc.description.affiliationInstituto de Biociências UNESP Universidade Estadual Paulista, São Paulo, Bauru
dc.description.affiliationCondensed Matter Physics Department Faculty of Science University of Valladolid, Valladolid
dc.description.affiliationUnespFaculdade de Ciências e Tecnologia UNESP Universidade Estadual Paulista, São Paulo, Presidente Prudente
dc.description.affiliationUnespFaculdade de Ciências UNESP Universidade Estadual Paulista, São Paulo, Bauru
dc.description.affiliationUnespInstituto de Biociências UNESP Universidade Estadual Paulista, São Paulo, Bauru
dc.format.extent1284-1293
dc.identifierhttp://dx.doi.org/10.1002/jbm.b.32941
dc.identifier.citationJournal of Biomedical Materials Research - Part B Applied Biomaterials, v. 101, n. 7, p. 1284-1293, 2013.
dc.identifier.doi10.1002/jbm.b.32941
dc.identifier.issn1552-4973
dc.identifier.issn1552-4981
dc.identifier.lattes9040459582368021
dc.identifier.orcid0000-0001-5389-0105
dc.identifier.orcid0000-0003-0162-8273
dc.identifier.scopus2-s2.0-84884204332
dc.identifier.urihttp://hdl.handle.net/11449/76677
dc.identifier.wosWOS:000324302100020
dc.language.isoeng
dc.relation.ispartofJournal of Biomedical Materials Research Part B: Applied Biomaterials
dc.relation.ispartofjcr3.373
dc.relation.ispartofsjr0,715
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectbiomaterial
dc.subjectcytotoxicity
dc.subjectpiezoelectricity
dc.subjectPVDF
dc.subjecttissue reaction
dc.subjectFourier transform infrared absorption spectroscopy
dc.subjectMononuclear leukocytes
dc.subjectMorphological changes
dc.subjectPolymorphonuclear leukocytes
dc.subjectPolyvinylidene fluorides
dc.subjectScanning electron microscopy image
dc.subjectTissue reactions
dc.subjectBiomaterials
dc.subjectCell culture
dc.subjectCollagen
dc.subjectCrystallography
dc.subjectCytotoxicity
dc.subjectFluorine compounds
dc.subjectMusculoskeletal system
dc.subjectPiezoelectricity
dc.subjectRubber applications
dc.subjectScanning electron microscopy
dc.subjectStarch
dc.subjectTissue
dc.subjectcollagen fiber
dc.subjectpolymer
dc.subjectpolyvinylidene fluoride
dc.subjectanimal cell
dc.subjectbone regeneration
dc.subjectcontrolled study
dc.subjectcytotoxicity test
dc.subjectimplantation
dc.subjectin vitro study
dc.subjectin vivo study
dc.subjectinflammatory cell
dc.subjectinfrared spectroscopy
dc.subjectmale
dc.subjectmononuclear cell
dc.subjectmorphometrics
dc.subjectmouse
dc.subjectneutrophil
dc.subjectnonhuman
dc.subjectpolymorphonuclear cell
dc.subjectrat
dc.subjectscanning electron microscopy
dc.subjectsubcutaneous tissue
dc.titleSubcutaneous tissue reaction and cytotoxicity of polyvinylidene fluoride and polyvinylidene fluoride-trifluoroethylene blends associated with natural polymersen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.author.lattes9040459582368021
unesp.author.lattes5268607684223281[7]
unesp.author.lattes6118325967319836[8]
unesp.author.orcid0000-0003-0162-8273[7]
unesp.author.orcid0000-0001-5389-0105[10]
unesp.author.orcid0000-0002-5921-3161[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Tecnologia, Presidente Prudentept

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