In vitro efficacy of the essential oil of Piper cubeba L. (Piperaceae) against Schistosoma mansoni

Página do item simplificado
dc.contributor.authorMagalhaes, Lizandra G.
dc.contributor.authorde Souza, Julia M.
dc.contributor.authorWakabayashi, Kamila A. L.
dc.contributor.authorLaurentiz, Rosangela da S. [UNESP]
dc.contributor.authorVinholis, Adriana H. C.
dc.contributor.authorRezende, Karen C. S.
dc.contributor.authorSimaro, Guilherme V.
dc.contributor.authorBastos, Jairo K.
dc.contributor.authorRodrigues, Vanderlei
dc.contributor.authorEsperandim, Viviane R.
dc.contributor.authorFerreira, Daniele S.
dc.contributor.authorCrotti, Antonio E. M.
dc.contributor.authorCunha, Wilson R.
dc.contributor.authorSilva, Marcio L. A. E.
dc.contributor.institutionFaculdade Medicina Ribeiro Preto
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFaculdade Farm Ribeiro Preto
dc.contributor.institutionUniv Franca
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:29:50Z
dc.date.available2014-05-20T13:29:50Z
dc.date.issued2012-05-01
dc.description.abstractIn this paper, cercariae, schistosomula, and adult Schistosoma mansoni worms were incubated in vitro with the essential oil of Piper cubeba (PC-EO) at concentrations from 12.5 to 200 mu g/mL, and the viability was evaluated using an inverted microscopy. The effects of PC-EO at 100 and 200 mu g/mL on the stages of S. mansoni were similar to those of the positive control (PZQ at 12.5 mu g/mL), with total absence of mobility after 120 h. However, at concentrations from 12.5 to 50 mu g/mL, PC-EO caused a reduction in the viability of cercariae and schistosomula when compared with the negative control groups (RPMI 1640 or dechlorinated water) or (RPMI 1640 + 0.1% DMSO or dechlorinated water + 0.1% DMSO). on the other hand, adult S. mansoni worms remained normally active when incubated with PC-EO at concentrations of 12.5 and 25 mu g/mL, and their viabilities were similar to those of the negative control groups. In addition, at concentrations ranging from 50 to 200 mu g/mL, separation of all the coupled adult worms was observed after 24 h of incubation, which is related to the fact of the reduction in egg production at this concentration. The main chemical constituents of PC-EO were identified by gas chromatography-mass spectrometry as being sabinene (19.99%), eucalyptol (11.87%), 4-terpineol (6.36%), beta-pinene (5.81%), camphor (5.61%), and delta-3-carene (5.34%). The cytotoxicity of the PC-EO was determined, and a significant cytotoxicity was only obtained in the concentration of 200 mu g/mL after 24 h treatment. The results suggest that PC-EO possesses an effect against cercariae, schistosomula, and adult worms of the S. mansoni.en
dc.description.affiliationFaculdade Medicina Ribeiro Preto, Univ So Paulo, Dept Bioquim Imunologia, Ribeirao Preto, SP, Brazil
dc.description.affiliationFaculdade Engenharia Ilha Solteira, Univ Estadual Paulista, Dept Quim Fis, Avenida Brasil 56, Ilha Solteira, SP, Brazil
dc.description.affiliationFaculdade Farm Ribeiro Preto, Univ So Paulo, Dept Cincias Farmacut, Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Franca, Nucleo Pesquisas Ciencias Exatas & Tecnol, BR-14404600 Franca, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Bioquim & Imunol, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Ciencias Farmaceut, Fac Farm Ribeirao Preto, BR-14030000 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Quim & Fis, Fac Engn Ilha Solteira, BR-15385000 Ilha Solteira, SP, Brazil
dc.description.affiliationUnespFaculdade Engenharia Ilha Solteira, Univ Estadual Paulista, Dept Quim Fis, Avenida Brasil 56, Ilha Solteira, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Quim & Fis, Fac Engn Ilha Solteira, BR-15385000 Ilha Solteira, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 09/15207-4
dc.description.sponsorshipIdFAPESP: 07/54241-8
dc.format.extent1747-1754
dc.identifierhttp://dx.doi.org/10.1007/s00436-011-2695-7
dc.identifier.citationParasitology Research. New York: Springer, v. 110, n. 5, p. 1747-1754, 2012.
dc.identifier.doi10.1007/s00436-011-2695-7
dc.identifier.issn0932-0113
dc.identifier.urihttp://hdl.handle.net/11449/10109
dc.identifier.wosWOS:000302814700020
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofParasitology Research
dc.relation.ispartofjcr2.558
dc.relation.ispartofsjr0,991
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleIn vitro efficacy of the essential oil of Piper cubeba L. (Piperaceae) against Schistosoma mansonien
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Engenharia, Ilha Solteirapt

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Física e Química - FEIS