Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells

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dc.contributor.authorScheffel, Débora Lopes Salles [UNESP]
dc.contributor.authorSoares, Diana Gabriela [UNESP]
dc.contributor.authorBasso, Fernanda Gonçalves [UNESP]
dc.contributor.authorCosta, Carlos Alberto de Souza
dc.contributor.authorPashley, David [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionGeorgia Regents University
dc.date.accessioned2015-12-07T15:32:22Z
dc.date.available2015-12-07T15:32:22Z
dc.date.issued2015
dc.description.abstractThis study investigated the transdentinal cytotoxicity of glutahaldehyde-containing solutions/materials on odontoblast-like cells. Dentin discs were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal side of the discs and the occlusal surface was treated with the following solutions: water, 2% glutaraldehyde (GA), 5% GA, 10% GA, Gluma Comfort Bond+Desensitizer (GCB+De) or Gluma Desensitizer (GDe). Cell viability and morphology were assessed by the Alamar Blue assay and SEM. The eluates were collected and applied on cells seeded in 24-well plates. After 7 or 14 days the total protein (TP) production, alkaline phosphatase activity (ALP) and deposition of mineralized nodules (MN) were evaluated. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (p<0.05). GA solutions were not cytotoxic against MDPC-23. GCB+De (85.1%) and GDe (77.2%) reduced cell viability as well as TP production and ALP activity at both periods. After 14 days, GCB+De and GDe groups produced less MN. Affected MDPC-23 presented deformation of the cytoskeleton and reduction of cellular projections. The treatment with 2.5%, 5% and 10% GA was not harmful to odontoblast-like cells. Conversely, when GA was combined with other components like HEMA, the final material became cytotoxic. Glutaraldehyde has been used to decrease dentin hypersensitivity. This substance is also capable of preventing resin-dentin bond degradation by cross-linking collagen and MMPs. This study showed that GA might be safe when applied on acid etched dentin. However, when combined with HEMA the product becomes cytotoxic.en
dc.description.affiliationDepartment of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, UNESP-Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil.
dc.description.affiliationDepartment of Dental Materials and Prosthodontics, Araraquara School of Dentistry, UNESP-Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil.
dc.description.affiliationDepartment of Physiology and Pathology, Araraquara School of Dentistry, UNESP-Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil.
dc.description.affiliationDepartment of Oral Biology, College of Dental Medicine, Georgia Regents University, 1120 15th Street, CL-2112, Augusta, Georgia 30912-1129, USA.
dc.description.affiliationDepartment of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, UNESP-Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil. Electronic address: jhebling@foar.unesp.br.
dc.description.affiliationUnespDepartment of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, UNESP-Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil.
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics, Araraquara School of Dentistry, UNESP-Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil.
dc.description.affiliationUnespDepartment of Physiology and Pathology, Araraquara School of Dentistry, UNESP-Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil.
dc.description.affiliationUnespDepartment of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, UNESP-Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil. Electronic address: jhebling@foar.unesp.br.
dc.format.extent997-1006
dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2015.05.004
dc.identifier.citationJournal Of Dentistry, v. 43, n. 8, p. 997-1006, 2015.
dc.identifier.doi10.1016/j.jdent.2015.05.004
dc.identifier.issn1879-176X
dc.identifier.lattes4517484241515548
dc.identifier.pmcPMC4509972
dc.identifier.pubmed25985981
dc.identifier.urihttp://hdl.handle.net/11449/131184
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofJournal Of Dentistry
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectCytotoxicityen
dc.subjectDentinen
dc.subjectGlutaraldehydeen
dc.subjectHydroxyethylmetacrylateen
dc.subjectOdontoblastsen
dc.titleTransdentinal cytotoxicity of glutaraldehyde on odontoblast-like cellsen
dc.typeArtigo
dcterms.rightsHolderElsevier B. V.
unesp.author.lattes4517484241515548[4]
unesp.author.orcid0000-0002-7455-6867[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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Artigos - Clínica Infantil - FOAR
Artigos - Fisiologia e Patologia - FOAR
Artigos - Materiais Odontológicos e Prótese - FOAR