Overlapped Sequence Types (STs) and Serogroups of Avian Pathogenic (APEC) and Human Extra-Intestinal Pathogenic (ExPEC) Escherichia coli Isolated in Brazil

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dc.contributor.authorMaluta, Renato Pariz
dc.contributor.authorLogue, Catherine Mary
dc.contributor.authorTiba Casas, Monique Ribeiro
dc.contributor.authorMeng, Ting
dc.contributor.authorLopes Guastalli, Elisabete Aparecida
dc.contributor.authorGalvao Rojas, Thais Cabrera
dc.contributor.authorMontelli, Augusto Cezar [UNESP]
dc.contributor.authorSadatsune, Terue [UNESP]
dc.contributor.authorRamos, Marcelo de Carvalho
dc.contributor.authorNolan, Lisa Kay
dc.contributor.authorSilveira, Wanderley Dias da
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionIowa State Univ
dc.contributor.institutionAdolfo Lutz Inst
dc.contributor.institutionCAPTAA
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:56:02Z
dc.date.available2015-03-18T15:56:02Z
dc.date.issued2014-08-12
dc.description.abstractAvian pathogenic Escherichia coli (APEC) strains belong to a category that is associated with colibacillosis, a serious illness in the poultry industry worldwide. Additionally, some APEC groups have recently been described as potential zoonotic agents. In this work, we compared APEC strains with extraintestinal pathogenic E. coli (ExPEC) strains isolated from clinical cases of humans with extra-intestinal diseases such as urinary tract infections (UTI) and bacteremia. PCR results showed that genes usually found in the ColV plasmid (tsh, iucA, iss, and hlyF) were associated with APEC strains while fyuA, irp-2, fepC sitD(chrom), fimH, crl, csgA, afa, iha, sat, hlyA, hra, cnf1, kpsMTII, clpV(Sakai) and malX were associated with human ExPEC. Both categories shared nine serogroups (O2, O6, O7, O8, O11, O19, O25, O73 and O153) and seven sequence types (ST10, ST88, ST93, ST117, ST131, ST155, ST359, ST648 and ST1011). Interestingly, ST95, which is associated with the zoonotic potential of APEC and is spread in avian E. coli of North America and Europe, was not detected among 76 APEC strains. When the strains were clustered based on the presence of virulence genes, most ExPEC strains (71.7%) were contained in one cluster while most APEC strains (63.2%) segregated to another. In general, the strains showed distinct genetic and fingerprint patterns, but avian and human strains of ST359, or ST23 clonal complex (CC), presented more than 70% of similarity by PFGE. The results demonstrate that some "zoonotic-related'' STs (ST117, ST131, ST10CC, ST23CC) are present in Brazil. Also, the presence of moderate fingerprint similarities between ST359 E. coli of avian and human origin indicates that strains of this ST are candidates for having zoonotic potential.en
dc.description.affiliationState Univ Campinas UNICAMP, Inst Biol, Dept Genet Evolut & Bioagents, Bacterial Mol Biol Lab, Campinas, SP, Brazil
dc.description.affiliationIowa State Univ, Coll Vet Med, Dept Vet Microbiol & Prevent Med, Ames, IA USA
dc.description.affiliationAdolfo Lutz Inst, Sao Paulo, Brazil
dc.description.affiliationCAPTAA, Unidade Pesquisa & Desenvolvimento Bastos, Inst Biol, Bastos, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Med, Dept Clin Med, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Microbiol & Imunol, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Internal Med, Campinas, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med, Dept Clin Med, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Microbiol & Imunol, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipDean's Office College of Veterinary Medicine, Iowa State University (ISU)
dc.description.sponsorshipVPR Office, ISU
dc.description.sponsorshipIdFAPESP: 12/05073-3
dc.description.sponsorshipIdFAPESP: 13/09167-5
dc.description.sponsorshipIdCAPES: PNPD/CAPES 23038.042588/2008-11
dc.description.sponsorshipIdFAPESP: 12/04931-6
dc.format.extent11
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0105016
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 9, n. 8, 11 p., 2014.
dc.identifier.doi10.1371/journal.pone.0105016
dc.identifier.fileWOS000341230400114.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11449/117398
dc.identifier.wosWOS:000341230400114
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleOverlapped Sequence Types (STs) and Serogroups of Avian Pathogenic (APEC) and Human Extra-Intestinal Pathogenic (ExPEC) Escherichia coli Isolated in Brazilen
dc.typeArtigo
dcterms.rightsHolderPublic Library Science
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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