Nutritional stress enhances cell viability of odontoblast-like cells subjected to low level laser irradiation

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dc.contributor.authorTagliani, M. M. [UNESP]
dc.contributor.authorOliveira, C. F. [UNESP]
dc.contributor.authorLins, E. M. M.
dc.contributor.authorKurachi, C.
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorBagnato, V. S.
dc.contributor.authorde Souza Costa, C. A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:46:02Z
dc.date.available2014-05-20T13:46:02Z
dc.date.issued2010-03-01
dc.description.abstractIn spite of knowing that cells tinder stress are biostimulated by low level laser (LLL) irradiation, the ideal condition of stress to different cell lines has not yet been established. Consequently, the aim of the present in vitro study was to evaluate the effects of a defined parameter of LLL irradiation applied on stressed odontoblast-like pulp cells (MDPC-23). The cells were seeded (12500 cells/cm(2)) in wells of 24-well plates using complete culture medium (DMEM) and incubated for 24 hours. Then, the DMEM was replaced by a new medium with low concentrations (nutritional stress condition) of fetal bovine serum (FBS) giving rise to the following experimental groups: G1: 2% FBS; G2: 5% FBS; and G3: 10% FBS. The cells were irradiated three times with LLL in specific parameters (808 +/- 3 nm, 100 mW, 1.5 J/cm(2)) every 24 hours. No irradiation was carried out in groups G4 (2% FBS-Control), G5 (5% FBS-Control), and G6 (10% FBS-Control). For all groups, the cell metabolism (MTT assay) and morphology (SEM) was evaluated. The experimental groups showed enhanced cell metabolism and normal cell morphology regardless of FBS concentration. A slight increase in the cell metabolism was observed only in group G2. It was concluded that cell nutritional stress caused by reducing the concentration of FBS to 5% is the most suitable method to assess the biostimulation of LLL irradiated MDPC-23 cells.[GRAPHICS]MDPC-23 cells adhered to the glass substrate exhibited a spindle-shaped morphology and some mitosis were observed (arrows); SEM x500 (C) 2010 by Astro Ltd. Published exclusively by WILEY-VCH Verlag GmbH & Co. KGaAen
dc.description.affiliationUNESP, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Phys, BR-13560970 São Carlos, SP, Brazil
dc.description.affiliationUnespUNESP, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 07/50646-3
dc.description.sponsorshipIdFAPESP: 08/08424-6
dc.description.sponsorshipIdFAPESP: 08/54785-0
dc.description.sponsorshipIdCNPq: 476137/2006
dc.description.sponsorshipIdCNPq: 301029/2007-5
dc.format.extent247-251
dc.identifierhttp://dx.doi.org/10.1002/lapl.200910137
dc.identifier.citationLaser Physics Letters. Little Rock: Astro Ltd, v. 7, n. 3, p. 247-251, 2010.
dc.identifier.doi10.1002/lapl.200910137
dc.identifier.issn1612-2011
dc.identifier.lattes4517484241515548
dc.identifier.urihttp://hdl.handle.net/11449/16256
dc.identifier.wosWOS:000275525500013
dc.language.isoeng
dc.publisherAstro Ltd
dc.relation.ispartofLaser Physics Letters
dc.relation.ispartofjcr2.240
dc.relation.ispartofsjr0,818
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcell cultureen
dc.subjectnutritional deficiten
dc.subjectlaseren
dc.subjectodontoblastsen
dc.titleNutritional stress enhances cell viability of odontoblast-like cells subjected to low level laser irradiationen
dc.typeArtigo
dcterms.licensehttp://iopscience.iop.org/info/page/openaccess
dcterms.rightsHolderAstro Ltd
unesp.author.lattes4517484241515548[7]
unesp.author.orcid0000-0002-7455-6867[7]
unesp.author.orcid0000-0002-7967-6163[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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