In vitro PGF2 alpha production by endometrium and corpus luteum explants from pregnant and nonpregnant diestrus bitches and placental explants from pregnant bitches

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dc.contributor.authorLuz, Marcelo Rezende
dc.contributor.authorBertan, Claudia Maria
dc.contributor.authorBinelli, Mario
dc.contributor.authorLopes, Maria Denise [UNESP]
dc.contributor.institutionUniversidade Federal do Espírito Santo (UFES)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-02-26T17:26:40Z
dc.date.accessioned2014-05-20T13:41:39Z
dc.date.available2014-02-26T17:26:40Z
dc.date.available2014-05-20T13:41:39Z
dc.date.issued2006-10-01
dc.description.abstractTo better understand the process of slow luteal regression of the nonpregnant cycle in dogs and the acute luteolysis that occurs prepartum, the present study investigated in vitro PGF2 alpha production by the endometrium, corpus luteum and placental explants obtained at known times of the cycle from pregnant bitches (days 63, 64 and immediately postpartum; day 0 = estimated day of the ovulatory LH surge) and from nonpregnant diestrus bitches (approximately days 65, 75 and 85). Both basal PGF2 alpha production and its production in the presence of the protein kinase C (PKC) stimulator 12,13-phorbol dibutyrate (PDBu) were determined. For PDBu-supplemented incubations, mean PGF2 alpha production (pg/mL/mg/6 h) by endometrium explants of the nonpregnant bitches in late diestrus was highest on day 65 (205 +/- 87) and reduced to low levels (38 +/- 17 and 11 +/- 11) on days 75 and 85, respectively. The production by corpus luteum explants from these bitches was significantly less on day 65 (46 +/- 14) than that of the day 65 endometrium explants, and was slightly increased on day 85 (103 +/- 52). The corresponding mean PGF2 alpha production by the endometrium explants of pregnant bitches was on average much greater (i.e., two to three-fold) compared to nonpregnant bitches (P < 0.01) and involved high concentrations at day 64 (1523 +/- 467) and postpartum, compared to somewhat lower levels on day 63 (830 +/- 65); luteal PGF production (165 +/- 4) was also higher than in nonpregnant bitches around day 65. For pregnant bitches, PGF production per gram of tissue in the endometrium explants was greater than for the CL or placenta explants (180 +/- 37). Therefore, the endometrium of the pregnant bitch has an increased capability to produce PGF2a immediately prepartum, which on a tissue weight basis, exceeds that of either corpora lutea or the placenta. However, assuming a larger mass of placental tissue in vivo, we inferred that the placenta may contribute substantially to peripheral PGF concentrations. (c) 2006 Published by Elsevier B.V.en
dc.description.affiliationFed Univ Espirito Santo, Dept Anim Sci & Rural Econ, CCA, BR-29500000 Alegre, ES, Brazil
dc.description.affiliationUniv São Paulo, Dept Anim Reprod, CBRA, LFEM, BR-05508 São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Anim Reprod & Vet Radiol, Sch Vet Med & Anim Sci FMVZ, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Anim Reprod & Vet Radiol, Sch Vet Med & Anim Sci FMVZ, BR-18618000 Botucatu, SP, Brazil
dc.format.extent1442-1447
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2006.01.037
dc.identifier.citationTheriogenology. New York: Elsevier B.V., v. 66, n. 6-7, p. 1442-1447, 2006.
dc.identifier.doi10.1016/j.theriogenology.2006.01.037
dc.identifier.issn0093-691X
dc.identifier.lattes6666129914663018
dc.identifier.lattes5720162493397851
dc.identifier.orcid0000-0003-4211-0649
dc.identifier.urihttp://hdl.handle.net/11449/14433
dc.identifier.wosWOS:000240887300007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbitchpt
dc.subjectluteal regressionpt
dc.subjectPGF2 alphapt
dc.subjectPDBupt
dc.subjectparturitionpt
dc.titleIn vitro PGF2 alpha production by endometrium and corpus luteum explants from pregnant and nonpregnant diestrus bitches and placental explants from pregnant bitchesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes6666129914663018
unesp.author.lattes5720162493397851[2]
unesp.author.orcid0000-0002-5995-3944[3]
unesp.author.orcid0000-0003-4211-0649[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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