Characterisation of the chromosome fusions in Oreochromis karongae

dc.contributor.authorMota-Velasco, Jose C.
dc.contributor.authorFerreira, Irani Alves [UNESP]
dc.contributor.authorCioffi, Marcelo B.
dc.contributor.authorOcalewicz, Konrad
dc.contributor.authorCampos-Ramos, Rafael
dc.contributor.authorShirak, Andrey
dc.contributor.authorLee, Bo-Young
dc.contributor.authorMartins, Cesar [UNESP]
dc.contributor.authorPenman, David J.
dc.contributor.institutionUniv Stirling
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniv Warmia & Mazury Olsztyn
dc.contributor.institutionCtr Invest Biol Noroeste SC CIBNOR
dc.contributor.institutionAgr Res Org
dc.contributor.institutionUniversity of Maryland
dc.date.accessioned2014-05-20T13:52:13Z
dc.date.available2014-05-20T13:52:13Z
dc.date.issued2010-07-01
dc.description.abstractOreochromis karongae, one of the "chambo" tilapia species from Lake Malawi, has a karyotype of 2n = 38, making it one of the few species investigated to differ from the typical tilapia karyotype (2n = 44). The O. karongae karyotype consists of one large subtelocentric pair of chromosomes, four medium-sized pairs (three subtelocentric and one submetacentric) and 14 small pairs. The five largest pairs could be distinguished from each other on the basis of size, morphology and a series of fluorescence in situ hybridisation (FISH) probes. The largest pair is easily distinguished on the basis of size and a chromosome 1 (linkage group 3) bacterial artificial chromosome (BAC) FISH probe from Oreochromis niloticus. BAC clones from O. niloticus chromosome 2 (linkage group 7) hybridised to one of the medium-sized subtelocentric chromosome pairs (no. 5) of O. karongae, distinguishing the ancestral medium-sized pair from the three other medium-sized chromosome pairs (nos. 2, 3 and 4) that appear to have resulted from fusions. SATA repetitive DNA hybridised to the centromeres of all 19 chromosome pairs and also revealed the locations of the relic centromeres in the three fused pairs. Telomeric (TTAGGG)(n) repeats were identified in the telomeres of all chromosomes, and an interstitial telomeric site (ITS) was identified in three chromosomal pairs (no. 2, 3 and 4). Additionally, two ITS sites were identified in the largest chromosome pair (pair 1), confirming the origin of this chromosome from three ancestral chromosomes. SATA and ITS sites allowed the orientation of the fusions in pairs 2, 3 and 4, which all appear to have been in different orientations (q-q, p-q and p-p, respectively). One of these fusions (O. karongae chromosome pair no. 2) involves a small chromosome (equivalent to linkage group 1), which in O. niloticus carries the main sex-determining gene. 4',6-Diamidino-2-phenyloindole staining of the synaptonemal complex in male O. karongae revealed the presumptive positions of the kinetochores, which correspond well to the centromeric positions observed in the mitotic karyotype.en
dc.description.affiliationUniv Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland
dc.description.affiliationUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Morfol, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUniversidade Federal de São Carlos (UFSCar), Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, BR-13565905 São Carlos, SP, Brazil
dc.description.affiliationUniv Warmia & Mazury Olsztyn, Dept Ichthyol, Olsztyn, Poland
dc.description.affiliationCtr Invest Biol Noroeste SC CIBNOR, La Paz, Mexico
dc.description.affiliationAgr Res Org, Volcani Ctr, Inst Anim Sci, IL-50250 Bet Dagan, Israel
dc.description.affiliationUniv Maryland, Dept Biol, College Pk, MD 20742 USA
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Morfol, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología (CONACYT)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipResearch Council of Norway
dc.description.sponsorshipUnited States-Israel Binational Agricultural Research and Development (BARD) Fund
dc.description.sponsorshipIdBARD Fund: IS-3995-07
dc.format.extent575-586
dc.identifierhttp://dx.doi.org/10.1007/s10577-010-9141-z
dc.identifier.citationChromosome Research. Dordrecht: Springer, v. 18, n. 5, p. 575-586, 2010.
dc.identifier.doi10.1007/s10577-010-9141-z
dc.identifier.issn0967-3849
dc.identifier.lattes8858800699425352
dc.identifier.orcid0000-0003-3534-974X
dc.identifier.urihttp://hdl.handle.net/11449/18661
dc.identifier.wosWOS:000280125500005
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofChromosome Research
dc.relation.ispartofjcr2.909
dc.relation.ispartofsjr1,425
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjecttilapiaen
dc.subjectchamboen
dc.subjectMalawien
dc.subjectcichliden
dc.subjectFISHen
dc.subjectsynaptonemal complexen
dc.subjectCyp19ben
dc.titleCharacterisation of the chromosome fusions in Oreochromis karongaeen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes8858800699425352[8]
unesp.author.orcid0000-0003-3534-974X[8]
unesp.author.orcid0000-0001-7122-7542[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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