Development of a peptide ELISA for the diagnosis of Equine arteritis virus

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dc.contributor.authorErnesto Metz, German
dc.contributor.authorLorenzon, Esteban Nicolas [UNESP]
dc.contributor.authorSoledad Serena, Maria
dc.contributor.authorGerardo Corva, Santiago
dc.contributor.authorJavier Panei, Carlos
dc.contributor.authorDiaz, Silvina
dc.contributor.authorCilli, Eduardo Maffud [UNESP]
dc.contributor.authorGabriela Echeverria, Maria
dc.contributor.institutionNatl Univ La Plata
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionConsejo Nacl Invest Cient & Tecn
dc.contributor.institutionIGEVET CCT La Plata
dc.date.accessioned2015-03-18T15:53:38Z
dc.date.available2015-03-18T15:53:38Z
dc.date.issued2014-09-01
dc.description.abstractA peptide-based indirect ELISA was developed to detect antibodies against Equine arteritis virus (EAV). Two peptides for epitope C of protein GP5 and fragment E of protein M were designed, synthesized, purified and used as antigens either alone or combined. Ninety-two serum samples obtained from the 2010 Equine viral arteritis outbreak, analyzed previously by virus neutralization, were evaluated by the ELISA here developed. The best resolution was obtained using peptide GP5. The analysis of the inter- and intraplate variability showed that the assay was robust. The results allow concluding that this peptide-based ELISA is a good alternative to the OIE-prescribed virus neutralization test because it can be standardized between laboratories, can serve as rapid screening, can improve the speed of diagnosis of EAV-negative horses and can be particularly useful for routine surveillance in large populations. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.affiliationNatl Univ La Plata, Fac Vet Sci, La Plata, Buenos Aires, Argentina
dc.description.affiliationUniv Estadual Paulista, Araraquara UNESP, Inst Chem, Sao Paulo, Brazil
dc.description.affiliationConsejo Nacl Invest Cient & Tecn, CCT La Plata, RA-1033 Buenos Aires, DF, Argentina
dc.description.affiliationIGEVET CCT La Plata, La Plata, Buenos Aires, Argentina
dc.description.affiliationUnespUniv Estadual Paulista, Araraquara UNESP, Inst Chem, Sao Paulo, Brazil
dc.format.extent3-6
dc.identifierhttp://dx.doi.org/10.1016/j.jviromet.2014.04.018
dc.identifier.citationJournal Of Virological Methods. Amsterdam: Elsevier Science Bv, v. 205, p. 3-6, 2014.
dc.identifier.doi10.1016/j.jviromet.2014.04.018
dc.identifier.issn0166-0934
dc.identifier.lattes9424346762460416
dc.identifier.orcid0000-0002-4767-0904
dc.identifier.urihttp://hdl.handle.net/11449/116640
dc.identifier.wosWOS:000339145200002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal Of Virological Methods
dc.relation.ispartofjcr1.756
dc.relation.ispartofsjr0,858
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectSynthetic peptidesen
dc.subjectELISAen
dc.subjectEquine arteritis virusen
dc.titleDevelopment of a peptide ELISA for the diagnosis of Equine arteritis virusen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes9424346762460416
unesp.author.orcid0000-0002-4767-0904[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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