Glucose,cholesterol,total proteins and insulin-like growth factor typeI values in the follicular fluid of female river buffaloes (Bubalus bubalis)

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dc.contributor.authorGallego, Carlos
dc.contributor.authorPrado, Margarida [UNESP]
dc.contributor.authorCamargos, Aline
dc.contributor.authorSousa, Diego [UNESP]
dc.contributor.authorSouza, Maria Inês Lenz
dc.contributor.authorRamos, A. A. [UNESP]
dc.contributor.authorLandim, Fernanda da Cruz [UNESP]
dc.contributor.authorOba, Eunice
dc.contributor.authorJorge, André Mendes [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidad Federal de Mato Grosso (UFMT)
dc.date.accessioned2016-04-01T18:44:05Z
dc.date.available2016-04-01T18:44:05Z
dc.date.issued2012
dc.description.abstractTwenty six Murrah female river buffaloes, between 45 and 70 d post-partum, empty, multiparae, with an average live weight of 675 ± 56 kg, and average body condition of 3.5 points, in a 1 to 5 scale, were used to determine the concentrations of glucose, cholesterol, total protein and insulin-like growth factor type I(IGF-I) in the follicular fluid. The fluid was collected from dominant follicles, with diameters between 8 and 12 mm, by in vivo follicular aspiration. The oestrous cycle stage was not taken into account. The wave of follicular development was synchronized six days prior to the collection. Biochemical analyses of glucose and cholesterol were performed by the enzymatic colorimetric method with the utilization of commercial kits of Glicose (GOD-PAP) and Cholesterol (CHOD-PAP) (Kovalent), respectively. For the determination of total protein, the commercial kit total Protein (Kovalent), method Biuret, was employed. Readings were carried out through absorption spectrophotometry with visible light. Through the radioimmunoanalysis (RIA) technique the concentration of IGF-I was obtained using commercial kits of IRMA IGF-I (IMMUNOTECH). Descriptive statistics was used, by applying the PROC MEANS procedure of the SAS (2009) statistical package. Glucose concentrations (4.0 ± 0.75 mmol/L) and IGF-I (340 ± 129.83 ng/mL) showed higher values in female river buffaloes and dairy cows regarding those reported in other studies. However, cholesterol levels (0.51 ± 0.12 mmol/L) and total proteins (58.4 ± 4.43 g/L) were lower. Results indicate that there is a relationship between the concentration of biochemical indicators, the nutritional aspects, the diameter of the aspired follicles and the productive period.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Produção Animal, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Botucatu, Depto. Produção Animal, Lageado, CEP 18618-000, SP, Brasil
dc.description.affiliationUniversidad Federal de Mato Grosso. Campo Grande. Mato Grosso. Brasil
dc.description.affiliationInstituto de Ciencia Animal, Apartado Postal 24, San José de las Lajas, Mayabeque, Cuba
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Produção Animal, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Botucatu, Depto. Produção Animal, Lageado, CEP 18618-000, SP, Brasil
dc.format.extent365-368
dc.identifierhttp://www.ciencia-animal.org/cuban-journal-of-agricultural-science/articles/V46-N4-Y2012-P365-C-Gallego.pdf
dc.identifier.citationCuban Journal of Agricultural Science, v. 46, n. 4, p. 365-368, 2012.
dc.identifier.issn0864-0408
dc.identifier.lattes9224380586557664
dc.identifier.lattes8878595413452908
dc.identifier.lattes8456490300814833
dc.identifier.orcid0000-0003-0333-7437
dc.identifier.orcid0000-0002-2420-2550
dc.identifier.urihttp://hdl.handle.net/11449/137081
dc.language.isoeng
dc.relation.ispartofCuban Journal of Agricultural Science
dc.relation.ispartofsjr0,125
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectBiochemical profileen
dc.subjectFollicular fluiden
dc.subjectPost-partumen
dc.titleGlucose,cholesterol,total proteins and insulin-like growth factor typeI values in the follicular fluid of female river buffaloes (Bubalus bubalis)en
dc.typeArtigo
unesp.author.lattes9224380586557664(8)
unesp.author.lattes8878595413452908[9]
unesp.author.lattes8456490300814833[7]
unesp.author.orcid0000-0002-9079-198X[9]
unesp.author.orcid0000-0003-0333-7437(8)
unesp.author.orcid0000-0002-2420-2550[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentProdução Animalpt

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