Comparing label free electrochemical impedimetric and capacitive biosensing architectures

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dc.contributor.authorFernandes, Flavio C. B. [UNESP]
dc.contributor.authorSantos, Adriano [UNESP]
dc.contributor.authorMartins, Denise C. [UNESP]
dc.contributor.authorGoes, Marcio S. [UNESP]
dc.contributor.authorBueno, Paulo Roberto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:08:38Z
dc.date.available2014-12-03T13:08:38Z
dc.date.issued2014-07-15
dc.description.abstractThe transducer faradaic signals of molecularly receptive interfaces associated with specific target binding can be sensitively monitored by electrochemical impedance and/or capacitance spectroscopies. A comparative evaluation of both impedimetric (associated with charge transfer resistance) and capacitive (associated with faradaic density of states) approaches was undertaken using C-reactive protein (CRP) antigen and antibody interaction as biomolecular binding model. Aiming at constructing redox free (impedimetric) and redox tethered receptive (capacitive) interfaces engineered by self-assembly monolayer, CRP sensitivity and limit of detections were comparatively assessed regarding biosensor capabilities. Binding affinity constant between CRP and anti-CRP interfacial receptor sites were additionally evaluated by the Langmuir adsorption model. Both the impedimetric and capacitive approaches reported similar values of experimental analytical parameters albeit the latter was found to have the advantage of requiring no solution redox reporter thus making it highly suitable for use in multiplexing affinity arrays. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.affiliationSao Paulo State Univ, Univ Estadual Paulista, UNESP, Inst Chem, BR-14800900 Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Univ Estadual Paulista, UNESP, Inst Chem, BR-14800900 Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipSao Paulo State University (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent96-102
dc.identifierhttp://dx.doi.org/10.1016/j.bios.2014.01.044
dc.identifier.citationBiosensors & Bioelectronics. Oxford: Elsevier Advanced Technology, v. 57, p. 96-102, 2014.
dc.identifier.doi10.1016/j.bios.2014.01.044
dc.identifier.issn0956-5663
dc.identifier.lattes0477045906733254
dc.identifier.orcid0000-0003-2827-0208
dc.identifier.urihttp://hdl.handle.net/11449/111413
dc.identifier.wosWOS:000334086100016
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiosensors & Bioelectronics
dc.relation.ispartofjcr8.173
dc.relation.ispartofsjr2,373
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCapacitance spectroscopyen
dc.subjectElectrochemistry impedance spectroscopyen
dc.subjectBiosensoren
dc.subjectImmunosensoren
dc.subjectSurface engineeringen
dc.titleComparing label free electrochemical impedimetric and capacitive biosensing architecturesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes0477045906733254[5]
unesp.author.orcid0000-0003-2827-0208[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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