Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors

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dc.contributor.authorCorrea, Natássia C.R.
dc.contributor.authorKuasne, Hellen
dc.contributor.authorFaria, Jerusa A. Q. A.
dc.contributor.authorSeixas, Cica C. S.
dc.contributor.authorSantos, Iria G. D.
dc.contributor.authorAbreu, Francine B.
dc.contributor.authorNonogaki, Suely
dc.contributor.authorRocha, Rafael M.
dc.contributor.authorSilva, Gerluza Aparecida Borges
dc.contributor.authorGobbi, Helenice
dc.contributor.authorRogatto, Silvia Regina [UNESP]
dc.contributor.authorGoes, Alfredo M.
dc.contributor.authorGomes, Dawidson A
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionCIPE
dc.contributor.institutionA. C. Camargo Cancer Center
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:49Z
dc.date.available2014-05-27T11:28:49Z
dc.date.issued2013-04-01
dc.description.abstractBreast cancer is the most common type of cancer among women worldwide. Research using breast cancer cell lines derived from primary tumors may provide valuable additional knowledge regarding this type of cancer. Therefore, the aim of this study was to investigate the phenotypic profiles of MACL-1 and MGSO-3, the only Brazilian breast cancer cell lines available for comparative studies. We evaluated the presence of hormone receptors, proliferation, differentiation and stem cell markers, using immunohistochemical staining of the primary tumor, cultured cells and xenografts implanted in immunodeficient mice. We also investigated the ability of the cell lines to form colonies and copy number alterations by array comparative genomic hybridization. Histopathological analysis showed that the invasive primary tumor from which the MACL-1 cell line was derived, was a luminal A subtype carcinoma, while the ductal carcinoma in situ (DCIS) that gave rise to the MGSO-3 cell line was a HER2 subtype tumor, both showing different proliferation levels. The cell lines and the tumor xenografts in mice preserved their high proliferative potential, but did not maintain the expression of the other markers assessed. This shift in expression may be due to the selection of an 'establishment' phenotype in vitro. Whole-genome DNA evaluation showed a large amount of copy number alterations (CNAs) in the two cell lines. These findings render MACL-1 and MGSO-3 the first characterized Brazilian breast cancer cell lines to be potentially used for comparative research. © 2013 Spandidos Publications Ltd. All rights reserved.en
dc.description.affiliationDepartment of Biochemistry and Immunology Federal University of Minas Gerais, Belo Horizonte
dc.description.affiliationDepartment of Biological Sciences State University of Londrina, Londrina
dc.description.affiliationDepartment of Morphology Federal University of Minas Gerais, Belo Horizonte
dc.description.affiliationNeoGene Laboratory CIPE
dc.description.affiliationDepartment of Anatomic Pathology A.C. Camargo Hospital, S̃o Paulo
dc.description.affiliationDepartment of Anatomic Pathology Federal University of Minas Gerais, Belo Horizonte
dc.description.affiliationDepartment of Urology School of Medicine Paulista State University, Botucatu
dc.description.affiliationBiological Sciences Institute Department of Biochemistry and Immunology Federal University of Minas Gerais, Av. Antonio Carlos 6627, 31270-901 Belo Horizonte, Minas Gerais
dc.description.affiliationUnespDepartment of Urology School of Medicine Paulista State University, Botucatu
dc.format.extent1299-1307
dc.identifierhttp://dx.doi.org/10.3892/or.2013.2284
dc.identifier.citationOncology Reports, v. 29, n. 4, p. 1299-1307, 2013.
dc.identifier.doi10.3892/or.2013.2284
dc.identifier.file2-s2.0-84874722716.pdf
dc.identifier.issn1021-335X
dc.identifier.issn1791-2431
dc.identifier.lattes2259986546265579
dc.identifier.scopus2-s2.0-84874722716
dc.identifier.urihttp://hdl.handle.net/11449/75033
dc.identifier.wosWOS:000316510600006
dc.language.isoeng
dc.relation.ispartofOncology Reports
dc.relation.ispartofjcr2.976
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectArray comparative genomic hybridization
dc.subjectBreast cancer
dc.subjectCell line
dc.subjectImmunohistochemistry
dc.subjectPrimary tumor
dc.subjectTumor xenograft
dc.subjectagar
dc.subjectcell marker
dc.subjectepidermal growth factor receptor 2
dc.subjectgenomic DNA
dc.subjectglyceraldehyde 3 phosphate dehydrogenase
dc.subjecthormone receptor
dc.subjectmucin 1
dc.subjecttelomerase
dc.subjectanimal tissue
dc.subjectbreast cancer
dc.subjectcancer cell culture
dc.subjectcell differentiation
dc.subjectcell proliferation
dc.subjectcomparative genomic hybridization
dc.subjectcomparative study
dc.subjectcontrolled study
dc.subjectcopy number variation
dc.subjectgenomics
dc.subjecthistopathology
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunohistochemistry
dc.subjectintraductal carcinoma
dc.subjectMACL 1 cell
dc.subjectMGSO 3 cell
dc.subjectmouse
dc.subjectnonhuman
dc.subjectphenotype
dc.subjectprimary tumor
dc.subjectpriority journal
dc.subjectstem cell
dc.subjecttumor cells and cell lines
dc.subjecttumor xenograft
dc.subjectAnimals
dc.subjectBrazil
dc.subjectBreast Neoplasms
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectComparative Genomic Hybridization
dc.subjectFemale
dc.subjectHumans
dc.subjectMice
dc.subjectReceptor, erbB-2
dc.subjectXenograft Model Antitumor Assays
dc.titleGenomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumorsen
dc.typeArtigo
dcterms.licensehttp://www.spandidos-publications.com/pages/terms
unesp.author.lattes2259986546265579
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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