Organization of 5S rDNA in species of the fish Leporinus: two different genomic locations are characterized by distinct nontranscribed spacers

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dc.contributor.authorMartins, C.
dc.contributor.authorGaletti, P. M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2014-05-20T15:28:43Z
dc.date.available2014-05-20T15:28:43Z
dc.date.issued2001-10-01
dc.description.abstractTo address understanding the organization of the 5S rRNA multigene family in the fish genome, the nucleotide sequence and organization array of 5S rDNA were investigated in the genus Leporinus, a representative freshwater fish group of South American fauna. PCR, subgenomic library screening, genomic blotting, fluorescence in situ hybridization, and DNA sequencing were employed in this study. Two arrays of 5S rDNA were identified for all species investigated, one consisting of monomeric repeat units of around 200 bp and another one with monomers of 900 bp. These 5S rDNA arrays were characterized by distinct NTS sequences (designated NTS-I and NTS-II for the 200- and 900-bp monomers, respectively); however, their coding sequences were nearly identical. The 5S rRNA genes were clustered in two chromosome loci, a major one corresponding to the NTS-I sites and a minor one corresponding to the NTS-II sites. The NTS-I sequence was variable among Leporinus spp., whereas the NTS-II was conserved among them and even in the related genus Schizodon. The distinct 5S rDNA arrays might characterize two 5S rRNA gene subfamilies that have been evolving independently in the genome.en
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618000 São Paulo, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Dept Genet & Evolucao, BR-13565905 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618000 São Paulo, Brazil
dc.format.extent903-910
dc.identifierhttp://dx.doi.org/10.1139/gen-44-5-903
dc.identifier.citationGenome. Ottawa: Natl Research Council Canada, v. 44, n. 5, p. 903-910, 2001.
dc.identifier.doi10.1139/gen-44-5-903
dc.identifier.issn0831-2796
dc.identifier.lattes8858800699425352
dc.identifier.orcid0000-0003-3534-974X
dc.identifier.urihttp://hdl.handle.net/11449/38481
dc.identifier.wosWOS:000171015300019
dc.language.isoeng
dc.publisherNatl Research Council Canada
dc.relation.ispartofGenome
dc.relation.ispartofjcr1.892
dc.relation.ispartofsjr0,655
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subject5S rDNApt
dc.subject5S rRNA genept
dc.subjectnontranscribed spacerpt
dc.subjectLeporinuspt
dc.subjectfishpt
dc.titleOrganization of 5S rDNA in species of the fish Leporinus: two different genomic locations are characterized by distinct nontranscribed spacersen
dc.typeArtigo
dcterms.licensehttp://www.nrcresearchpress.com/page/authors/information/rights
dcterms.rightsHolderNatl Research Council Canada
unesp.author.lattes8858800699425352[1]
unesp.author.orcid0000-0003-3534-974X[1]
unesp.author.orcid0000-0001-5916-6126[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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