The promoter of a gene encoding an isoflavone reductase-like protein in coffee (Coffea arabica) drives a stress-responsive expression in leaves

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dc.contributor.authorBrandalise, Marcos [UNESP]
dc.contributor.authorSeverino, Fabio E. [UNESP]
dc.contributor.authorMaluf, Mirian P.
dc.contributor.authorMaia, Ivan de Godoy [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto Agronômico (IAC)
dc.date.accessioned2014-05-20T13:50:23Z
dc.date.available2014-05-20T13:50:23Z
dc.date.issued2009-11-01
dc.description.abstractA cDNA clone (designated CaIRL) encoding an isoflavone reductase-like protein from coffee (Coffea arabica) was retrieved during a search for genes showing organ/tissue-specific expression among the expressed sequence tags (EST) of the Brazilian coffee EST database. The CaIRL cDNA contains a single open reading frame of 946 nucleotides (nt) encoding 314 amino acids (predicted molecular weight of 34 kDa). Several features identified the predicted CaIRL protein as a new member of the PIP family of NADPH-dependent reductases. Expression studies demonstrated that CaIRL is expressed exclusively in coffee leaves and its transcript level is markedly increased in response to fungal infection and mechanical injury. Analysis of transgenic tobacco plants harboring a CaIRL 5'-flanking region (862 nt) fused to uidA reporter gene (GUS) confirmed the responsiveness of the putative promoter to abiotic stress in wounded leaves. In turn, a 5' deletion to -404 completely abolished promoter activation by abiotic stimulus in transgenic plants. The lack of GUS expression in non-wounded leaf tissues in transgenic tobacco was in contrast to the basal level of CaIRL expression observed in non-stressed healthy coffee leaves.en
dc.description.affiliationUNESP, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationInstituto Agronômico de Campinas, Ctr Cafe Alcides Carvalho, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Cafe, Campinas, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipConsorcio Brasileiro de Pesquisa e Desenvolvimento do Cafe (CBPD-Cafe)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent1699-1708
dc.identifierhttp://dx.doi.org/10.1007/s00299-009-0769-0
dc.identifier.citationPlant Cell Reports. New York: Springer, v. 28, n. 11, p. 1699-1708, 2009.
dc.identifier.doi10.1007/s00299-009-0769-0
dc.identifier.issn0721-7714
dc.identifier.lattes8649222099176162
dc.identifier.urihttp://hdl.handle.net/11449/17980
dc.identifier.wosWOS:000271905900009
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofPlant Cell Reports
dc.relation.ispartofjcr2.989
dc.relation.ispartofsjr1,291
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCoffeeen
dc.subjectPromoteren
dc.subjectTissue-specificityen
dc.subjectGene expressionen
dc.subjectIsoflavone-like proteinsen
dc.titleThe promoter of a gene encoding an isoflavone reductase-like protein in coffee (Coffea arabica) drives a stress-responsive expression in leavesen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes8649222099176162
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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