Antimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom

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dc.contributor.authorTorres, A. F. C.
dc.contributor.authorDantas, R. T.
dc.contributor.authorMenezes, R. R. P. P. B.
dc.contributor.authorToyama, M. H. [UNESP]
dc.contributor.authorDrigo Filho, Elso [UNESP]
dc.contributor.authorOliveira, M. F.
dc.contributor.authorNogueira, N. A. P.
dc.contributor.authorOliveira, M. R.
dc.contributor.authorMonteiro, H. S. A.
dc.contributor.authorMartins, A. M. C.
dc.contributor.institutionUniversidade Federal do Ceará (UFC)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal da Paraíba (UFPB)
dc.date.accessioned2014-05-20T14:02:26Z
dc.date.available2014-05-20T14:02:26Z
dc.date.issued2010-01-01
dc.description.abstractSome snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW (R) (Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 mu g/mL, which corresponded to its minimum lethal concentration (MLC). BleuTV also inhibited the growth of promastigote forms of L. chagasi and L. amazonensis, with respective IC(50) values of 1.94 mu g/mL and 5.49 mu g/mL. Furthermore, it repressed T. cruzi growth with an IC(50) of 1.14 mu g/mL. However, BleuLAAO did not inhibit the growth of the microorganisms studied and was not toxic to macrophages. BleuTV had low toxicity against macrophages at the concentrations studied. In conclusion, whole venom from Bothrops leucurus inhibited the growth of some microorganisms, including S. aureus, Leishmania sp., and T. cruzi.en
dc.description.affiliationUniversidade Federal do Ceará (UFC), Fac Farm, Dept Anal Clin & Toxicol, Sch Pharm, BR-60420970 Fortaleza, Ceara, Brazil
dc.description.affiliationUniversidade Federal do Ceará (UFC), Dept Physiol & Pharmacol, Fortaleza, Ceara, Brazil
dc.description.affiliationSão Paulo State Univ UNESP Univ Estadual Paulista, Lab Cell Biol & Chem Prot & Peptides, Sao Vicente, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, State Univ Campinas, Inst Biol, Dept Biochem, Campinas, SP, Brazil
dc.description.affiliationUniversidade Federal da Paraíba (UFPB), Ctr Exact Sci & Nat, Dept Mol Biol, BR-58059900 Joao Pessoa, Paraiba, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP Univ Estadual Paulista, Lab Cell Biol & Chem Prot & Peptides, Sao Vicente, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent614-622
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992010000400012
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 4, p. 614-622, 2010.
dc.identifier.fileS1678-91992010000400012-en.pdf
dc.identifier.issn1678-9199
dc.identifier.lattes8573195327542061
dc.identifier.scieloS1678-91992010000400012
dc.identifier.urihttp://hdl.handle.net/11449/22009
dc.identifier.wosWOS:000284778000012
dc.language.isoeng
dc.publisherUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofjcr1.782
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectL-amino oxidaseen
dc.subjectBothrops leucurusen
dc.subjectantimicrobial activityen
dc.titleAntimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venomen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/jvatitd/iaboutj.htm
dcterms.rightsHolderCevap-unesp
unesp.author.lattes8573195327542061
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept

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