Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst

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dc.contributor.authorOzawa, Manabu
dc.contributor.authorSakatani, Miki
dc.contributor.authorYao, JiQiang
dc.contributor.authorShanker, Savita
dc.contributor.authorYu, Fahong
dc.contributor.authorYamashita, Rui
dc.contributor.authorWakabayashi, Shunichi
dc.contributor.authorNakai, Kenta
dc.contributor.authorDobbs, Kyle B.
dc.contributor.authorSudano, Mateus Jose [UNESP]
dc.contributor.authorFarmerie, William G.
dc.contributor.authorHansen, Peter J.
dc.contributor.institutionUniv Florida
dc.contributor.institutionDH Barron Reprod & Perinatal Biol Res Program
dc.contributor.institutionNatl Agr & Food Res Org
dc.contributor.institutionUniv Tokyo
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2013-09-30T18:28:57Z
dc.date.accessioned2014-05-20T13:42:13Z
dc.date.available2013-09-30T18:28:57Z
dc.date.available2014-05-20T13:42:13Z
dc.date.issued2012-11-06
dc.description.abstractBackground: The first distinct differentiation event in mammals occurs at the blastocyst stage when totipotent blastomeres differentiate into either pluripotent inner cell mass (ICM) or multipotent trophectoderm (TE). Here we determined, for the first time, global gene expression patterns in the ICM and TE isolated from bovine blastocysts. The ICM and TE were isolated from blastocysts harvested at day 8 after insemination by magnetic activated cell sorting, and cDNA sequenced using the SOLiD 4.0 system.Results: A total of 870 genes were differentially expressed between ICM and TE. Several genes characteristic of ICM (for example, NANOG, SOX2, and STAT3) and TE (ELF5, GATA3, and KRT18) in mouse and human showed similar patterns in bovine. Other genes, however, showed differences in expression between ICM and TE that deviates from the expected based on mouse and human.Conclusion: Analysis of gene expression indicated that differentiation of blastomeres of the morula-stage embryo into the ICM and TE of the blastocyst is accompanied by differences between the two cell lineages in expression of genes controlling metabolic processes, endocytosis, hatching from the zona pellucida, paracrine and endocrine signaling with the mother, and genes supporting the changes in cellular architecture, stemness, and hematopoiesis necessary for development of the trophoblast.en
dc.description.affiliationUniv Florida, Dept Anim Sci, Gainesville, FL 32611 USA
dc.description.affiliationDH Barron Reprod & Perinatal Biol Res Program, Gainesville, FL 32611 USA
dc.description.affiliationUniv Florida, Interdisciplinary Ctr Biotechnol Res, Gainesville, FL 32611 USA
dc.description.affiliationNatl Agr & Food Res Org, Kyushu Okinawa Agr Res Ctr, Kumamoto, Japan
dc.description.affiliationUniv Tokyo, Inst Med Sci, Lab Dev Genet, Tokyo, Japan
dc.description.affiliationUniv Tokyo, Inst Med Sci, Ctr Human Genome, Tokyo, Japan
dc.description.affiliationUNESP, Fac Med Vet & Zootecn, Dept Reprod Anim & Radiol Vet, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Med Vet & Zootecn, Dept Reprod Anim & Radiol Vet, Botucatu, SP, Brazil
dc.description.sponsorshipAgriculture and Food Research Initiative from United States Dept. of Agriculture National Institute of Food and Agriculture
dc.description.sponsorshipResearch Opportunity Fund, University of Florida Research Foundation
dc.description.sponsorshipIdNIFA: 2009-65203-05732
dc.description.sponsorshipIdNIFA: 2011-67015-30688
dc.format.extent1-13
dc.identifierhttp://dx.doi.org/10.1186/1471-213X-12-33
dc.identifier.citationBmc Developmental Biology. London: Biomed Central Ltd., v. 12, p. 1-13, 2012.
dc.identifier.doi10.1186/1471-213X-12-33
dc.identifier.fileWOS000312128300001.pdf
dc.identifier.issn1471-213X
dc.identifier.urihttp://hdl.handle.net/11449/14679
dc.identifier.wosWOS:000312128300001
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofBmc Developmental Biology
dc.relation.ispartofjcr2.427
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectBlastocysten
dc.subjectTrophectodermen
dc.subjectInner cell massen
dc.subjectDevelopmenten
dc.titleGlobal gene expression of the inner cell mass and trophectoderm of the bovine blastocysten
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
unesp.author.orcid0000-0002-8721-8883[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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