Cytoplasmic RNA and nuclear changes detected cytochemically during the degeneration of salivary glands of the tick Rhipicephalus sanguineus (Latreille, 1806) (Acari, Ixodidae)

Página do item simplificado
dc.contributor.authorScopinho Furquim, Karim Christina [UNESP]
dc.contributor.authorBechara, Gervasio Henrique [UNESP]
dc.contributor.authorCamargo Mathias, Maria Izabel [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:55:10Z
dc.date.available2014-05-20T13:55:10Z
dc.date.issued2008-10-01
dc.description.abstractThe present study reports cytochemistry data about salivary glands of females (unfed, engorged, and at day three post-engorgement) and males (unfed, at day seven post-attachment, and at days three and seven post-detachment from the host) of the tick Rhipicephalus sanguineus. The results revealed nuclear changes in engorged females and at day three post-engorgement, and in males in all stages (except unfed). These changes were more prominent in females. Cytoplasmic changes were also observed in cells of all acini of males and females.In types II and III acini of engorged females, nuclear changes were observed in the shape (irregular, with blebs, fragmenting or fragmented), size (enlarged or reduced), and arrangement and condensation level of chromatin (marginal or as blebs). Changes were also detected in nucleoli, regarding their shape (fragmenting or fragmented), size (enlarged), and location (central, marginal or as blebs). Some nucleoli were also compacted or disorganized. In females at day three pos-enuorgement, all acini exhibited similar changes to those observed in engorged females. RNA staining was stronger in cells of engorged females than those at day three post-engorgement.In males at day seven post-attachment, cells of types II, III, IV acini presented changes in the size of the nucleus and condensation level of chromatin similar to those of females. The shape of the nucleus was round, irregular or undergoing fragmentation, and the chromatin was located at the margin or throughout the nucleus. The changes in the nucleolus were similar to those of females, regarding size and organization, although round-shaped and in the central location. In males at day three post-detachment, cells of all acini exhibited nuclear changes similar to those of males at day seven post-attachment, in addition to the fragmentation of the nucleolus. At day seven post-detachment. changes were detected in all acini similar to the observed in males at day seven post-attachment. Regarding cytoplasmic RNA, staining was prominent in males at day seven post-attachment and weak in those at day seven post-detachment from the host.In females as well as males, different RNA staining patterns in the cytoplasm and nuclear changes characterized apoptotic cell death. (C) 2007 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUNESP, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUNESP, FCAV, Dept Patol Vet, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, FCAV, Dept Patol Vet, BR-14884900 Jaboticabal, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipEuropean Union
dc.description.sponsorshipIdFAPESP: 05/59208-3
dc.description.sponsorshipIdEuropean Union: 510561-INCO
dc.format.extent960-966
dc.identifierhttp://dx.doi.org/10.1016/j.micron.2007.10.022
dc.identifier.citationMicron. Oxford: Pergamon-Elsevier B.V. Ltd, v. 39, n. 7, p. 960-966, 2008.
dc.identifier.doi10.1016/j.micron.2007.10.022
dc.identifier.issn0968-4328
dc.identifier.urihttp://hdl.handle.net/11449/19739
dc.identifier.wosWOS:000259422800026
dc.language.isoeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relation.ispartofMicron
dc.relation.ispartofjcr1.728
dc.relation.ispartofsjr0,624
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectRhipicephalus sanguineusen
dc.subjectSalivary glanden
dc.subjectfemalesen
dc.subjectmalesen
dc.subjectchromatinen
dc.subjectnucleolusen
dc.subjectApoptosisen
dc.titleCytoplasmic RNA and nuclear changes detected cytochemically during the degeneration of salivary glands of the tick Rhipicephalus sanguineus (Latreille, 1806) (Acari, Ixodidae)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderPergamon-Elsevier B.V. Ltd
unesp.author.orcid0000-0002-9418-8934[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Biologia - IBRC
Artigos - Patologia Veterinária - FCAV