Structural characterization of novel chemotactic and mastoparan peptides from the venom of the social wasp Agelaia pallipes pallipes by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry

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dc.contributor.authorMendes, Maria Anita [UNESP]
dc.contributor.authorSouza, Bibiana Monson de [UNESP]
dc.contributor.authorSantos, Lucilene Delazari dos [UNESP]
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:04Z
dc.date.available2014-05-27T11:21:04Z
dc.date.issued2004-04-15
dc.description.abstractHigh-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS) and high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) techniques were applied for the detection, purification, monitoring, and sequencing of two novel and biologically active peptides occurring at very low levels in the venom of the wasp Agelaia pallipes pallipes. These peptides were sequenced under LC/ESI-MS/MS conditions and designated as Agelaia-CP (I/L-L-G-T-I-L-G-L-L-K-G-I/L-NH2, MW 1207.8Da) and Agelaia-MP (I/L-N-W-L-K-L-G-K-A-I-I-D-A-I/L-NH2, MW 1565.0Da). The peptide Agelaia-CP showed no hemolytic activity, but it behaved as a mast cell degranulator and induced a potent chemotaxis in polymorphonucleated leukocyte (PMNL) cells, typical of a wasp chemotactic peptide. The peptide Agelaia-MP showed both powerful mast cell degranulation and hemolysis of washed rat red blood cells, and is thus assigned as a new member of the mastoparan family of peptides. Both peptides seem to be directly involved in the strong inflammatory reactions associated with wasp stings. © Copyright 2004 John Wiley & Sons, Ltd.en
dc.description.affiliationCEIS - Dept. of Biology/IBRC-UNESP Inst. of Immunol. Investigations Millennium Institute-MCT/CNPq, Rio Claro/SP 13506-900
dc.description.affiliationUnespCEIS - Dept. of Biology/IBRC-UNESP Inst. of Immunol. Investigations Millennium Institute-MCT/CNPq, Rio Claro/SP 13506-900
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent636-642
dc.identifierhttp://dx.doi.org/10.1002/rcm.1382
dc.identifier.citationRapid Communications in Mass Spectrometry, v. 18, n. 6, p. 636-642, 2004.
dc.identifier.doi10.1002/rcm.1382
dc.identifier.issn0951-4198
dc.identifier.lattes2901888624506535
dc.identifier.lattes3368404126695911
dc.identifier.scopus2-s2.0-1842585620
dc.identifier.urihttp://hdl.handle.net/11449/130709
dc.identifier.wosWOS:000220467000006
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofRapid Communications in Mass Spectrometry
dc.relation.ispartofjcr1.970
dc.relation.ispartofsjr0,632
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectChemotactic peptide
dc.subjectMastoparan
dc.subjectUnclassified drug
dc.subjectWasp venom
dc.subjectAgelaia pallipes pallipes
dc.subjectAnimal cell
dc.subjectBee sting
dc.subjectElectrospray mass spectrometry
dc.subjectHemolysis
dc.subjectHigh performance liquid chromatography
dc.subjectInflammation
dc.subjectMast cell degranulation
dc.subjectNeutrophil chemotaxis
dc.subjectNonhuman
dc.subjectProtein analysis
dc.subjectPprotein purification
dc.subjectProtein structure
dc.subjectRat
dc.subjectSequence analysis
dc.subjectTandem mass spectrometry
dc.subjectWasp
dc.subjectWasp sting
dc.subjectAnimals
dc.subjectChemotactic Factors
dc.subjectChromatography, High Pressure Liquid
dc.subjectMolecular Structure
dc.subjectPeptides
dc.subjectSequence Analysis, Protein
dc.subjectSpectrometry, Mass, Electrospray Ionization
dc.subjectWasp Venoms
dc.subjectWasps
dc.titleStructural characterization of novel chemotactic and mastoparan peptides from the venom of the social wasp Agelaia pallipes pallipes by high-performance liquid chromatography/electrospray ionization tandem mass spectrometryen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.advisor.lattes3368404126695911
unesp.author.lattes2901888624506535
unesp.author.orcid0000-0001-5832-1825[3]
unesp.author.orcid0000-0002-2078-9286[1]
unesp.author.orcid0000-0002-7363-8211[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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