The dead core model applied to beads with immobilized cells in a fed-batch cephalosporin C production bioprocess

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dc.contributor.authorCruz, AJG
dc.contributor.authorAlmeida, RMRG
dc.contributor.authorAraujo, MLGC
dc.contributor.authorGiordano, R. C.
dc.contributor.authorHokka, C. O.
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:24:03Z
dc.date.available2014-05-20T15:24:03Z
dc.date.issued2001-01-01
dc.description.abstractViable cells immobilized in inert supports are currently studied for a wide range of bioprocesses. The intrinsic advantages of such systems over suspended cultures incite new research, including studies on fundamental aspects as well as on the industrial viability of these non-conventional processes. In aerobic culture of filamentous fungi, scale-up is hindered by oxygen mass transfer limitation through the support material and bioprocess kinetics must be studied together with mass transfer limitation. In this work, experimental and simulated data of cephalosporin C production were compared. Concentrations in the bulk fermentation medium and cellular mass profiles inside the bioparticles are focused. Immobilized cells were used in a tower bioreactor, operated in fed-batch mode. To describe the radial variation of oxygen concentration within the pellet, a dead core model was used. Despite the extremely low sugar concentrations, bioreaction rates in the pellets were limited by the dissolved oxygen concentration. Cell growth occurs only in the outer layers, a result also confirmed by scanning electron microscopy. (C) 2001 Elsevier B.V. Ltd. All rights reserved.en
dc.description.affiliationUniv Fed Sao Carlos, Dept Engn Quim, BR-13565905 Sao Carlos, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Dept Tecnol, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Dept Tecnol, BR-14801970 Araraquara, SP, Brazil
dc.format.extent419-425
dc.identifierhttp://dx.doi.org/10.1016/S0009-2509(00)00244-X
dc.identifier.citationChemical Engineering Science. Oxford: Pergamon-Elsevier B.V., v. 56, n. 2, p. 419-425, 2001.
dc.identifier.doi10.1016/S0009-2509(00)00244-X
dc.identifier.issn0009-2509
dc.identifier.urihttp://hdl.handle.net/11449/34710
dc.identifier.wosWOS:000167291200018
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofChemical Engineering Science
dc.relation.ispartofjcr3.306
dc.relation.ispartofsjr1,043
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcephalosporin Cpt
dc.subjectbeta-lactampt
dc.subjectCephalosporium acremoniumpt
dc.subjectdead core modelpt
dc.subjectfed-batchpt
dc.titleThe dead core model applied to beads with immobilized cells in a fed-batch cephalosporin C production bioprocessen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

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