Tuberculosis survey of free-ranging marsh deer (Blastocerus dichotomus) in Brazil

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dc.contributor.authorLuna, Janaina O.
dc.contributor.authorSantos, Manoel A. A.
dc.contributor.authorDurigon, Edison L.
dc.contributor.authorAraújo, João P. [UNESP]
dc.contributor.authorDuarte, José M. B. [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:20:57Z
dc.date.available2014-05-27T11:20:57Z
dc.date.issued2003-12-01
dc.description.abstractEsophageal-pharyngeal fluids from 53 free-ranging marsh deer (Blastocerus dichotomus) captured for a research program in the state of Mato Grosso do Sul, Brazil, were assayed for tuberculosis. Total DNA was extracted, amplified by polymerase chain reaction using specific primers for Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis, M. microti, and M. africanum), and observed by agarose gel electrophoresis stained with ethidium bromide. All samples were negative. This, along with necropsy and histopathology data, suggests that these animals are not shedding and probably do not have active disease.en
dc.description.affiliationLab. Biol. Molec. Apl. e Sorologia Depto. de Med. Vet. Preventiva Universidade de Säo Paulo, Av. Orlando Marques de Paiva, 87, Sao Paulo City, Sao Paulo 05508-000
dc.description.affiliationInst. de Cie. Biomédicas Universidade de São Paulo, Caixa Postal 66.028, Sao Paulo City, Sao Paulo 05389-970
dc.description.affiliationDepto. de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista, Caixa Postal 510, Botucatu, São Paulo 18618-000
dc.description.affiliationDepartamento de Zootecnia Faculdade Cie. Agrarias Veterinarias Universidade Estadual Paulista, Via A. Prof. P. D. Castellane s/n, Jaboticabal, Sao Paulo 14870-000
dc.description.affiliationUnespDepto. de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista, Caixa Postal 510, Botucatu, São Paulo 18618-000
dc.description.affiliationUnespDepartamento de Zootecnia Faculdade Cie. Agrarias Veterinarias Universidade Estadual Paulista, Via A. Prof. P. D. Castellane s/n, Jaboticabal, Sao Paulo 14870-000
dc.format.extent414-415
dc.identifierhttp://dx.doi.org/10.1638/02-068
dc.identifier.citationJournal of Zoo and Wildlife Medicine, v. 34, n. 4, p. 414-415, 2003.
dc.identifier.doi10.1638/02-068
dc.identifier.issn1042-7260
dc.identifier.scopus2-s2.0-1642309574
dc.identifier.urihttp://hdl.handle.net/11449/67522
dc.identifier.wosWOS:000220220400014
dc.language.isoeng
dc.relation.ispartofJournal of Zoo and Wildlife Medicine
dc.relation.ispartofjcr0.684
dc.relation.ispartofsjr0,424
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectBlastocerus dichotomus
dc.subjectMarsh deer
dc.subjectMycobacterium bovis
dc.subjectPCR
dc.subjectTuberculosis
dc.subjectbacterial DNA
dc.subjectanimal
dc.subjectanimal disease
dc.subjectBrazil
dc.subjectdeer
dc.subjectgenetics
dc.subjectisolation and purification
dc.subjectmethodology
dc.subjectmicrobiology
dc.subjectMycobacterium
dc.subjectpolymerase chain reaction
dc.subjecttuberculosis
dc.subjectwild animal
dc.subjectAnimals
dc.subjectAnimals, Wild
dc.subjectDeer
dc.subjectDNA, Bacterial
dc.subjectPolymerase Chain Reaction
dc.subjectAnimalia
dc.subjectBacteria (microorganisms)
dc.subjectCaesalpinia ciliata
dc.subjectCervidae
dc.subjectMycobacterium africanum
dc.subjectMycobacterium microti
dc.subjectMycobacterium tuberculosis
dc.titleTuberculosis survey of free-ranging marsh deer (Blastocerus dichotomus) in Brazilen
dc.typeArtigo
dcterms.licensehttp://www.bioone.org/page/terms_of_use
unesp.author.orcid0000-0002-9153-1485[4]
unesp.author.orcid0000-0002-7805-0265[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Microbiologia e Imunologia - IBB
Artigos - Zootecnia - FCAV