Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats

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dc.contributor.authorde Moura, Leandro Pereira [UNESP]
dc.contributor.authorSouza Pauli, Luciana Santos
dc.contributor.authorCintra, Dennys Esper
dc.contributor.authorde Souza, Claudio Teodoro
dc.contributor.authorda Silva, Adelino Sanchez Ramos
dc.contributor.authorMarinho, Rodolfo
dc.contributor.authorde Melo, Maria Alice Rostom [UNESP]
dc.contributor.authorRopelle, Eduardo Rochete
dc.contributor.authorPauli, José Rodrigo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionLaboratório de Bioquímica e Fisiologia
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:28:33Z
dc.date.available2014-05-27T11:28:33Z
dc.date.issued2013-02-25
dc.description.abstractIt is now commonly accepted that chronic inflammation associated with obesity during aging induces insulin resistance in the liver. In the present study, we investigated whether the improvement in insulin sensitivity and insulin signaling, mediated by acute exercise, could be associated with modulation of protein-tyrosine phosphatase 1B (PTP-1B) in the liver of old rats. Aging rats were subjected to swimming for two 1.5-h long bouts, separated by a 45 min rest period. Sixteen hours after the exercise, the rats were sacrificed and proteins from the insulin signaling pathway were analyzed by immunoblotting. Our results show that the fat mass was increased in old rats. The reduction in glucose disappearance rate (Kitt) observed in aged rats was restored 16 h after exercise. Aging increased the content of PTP-1B and attenuated insulin signaling in the liver of rats, a phenomenon that was reversed by exercise. Aging rats also increased the IRβ/PTP-1B and IRS-1/PTP-1B association in the liver when compared with young rats. Conversely, in the liver of exercised old rats, IRβ/PTP-1B and IRS-1/PTP-1B association was markedly decreased. Moreover, in the hepatic tissue of old rats, the insulin signalling was decreased and PEPCK and G6Pase levels were increased when compared with young rats. Interestingly, 16 h after acute exercise, the PEPCK and G6Pase protein level were decreased in the old exercised group. These results provide new insights into the mechanisms by which exercise restores insulin signalling in liver during aging. © 2013 Moura et al; licensee BioMed Central Ltd.en
dc.description.affiliationUniversidade Estadual Paulista UNESP Curso de Pós-Graduação em Ciências da Motricidade Humana, Rio Claro, SP
dc.description.affiliationFaculdade de Ciências Aplicadas Universidade Estadual de Campinas Curso de Pós-Graduação em Nutrição, Esporte e Metabolismo. UNICAMP, Limeira, SP
dc.description.affiliationUniversidade do Extremo Sul Catarinense Laboratório de Bioquímica e Fisiologia, Santa Catarina, Criciúma, SC
dc.description.affiliationUniversidade de São Paulo Escola de Educação Física e Esporte USP, Ribeirão Preto, SP
dc.description.affiliationCurso de Ciências do Esporte FCA-UNICAMP, Rua Pedro Zaccaria, 1300, Jardim Santa Luzia, Limeira, SP
dc.description.affiliationUnespUniversidade Estadual Paulista UNESP Curso de Pós-Graduação em Ciências da Motricidade Humana, Rio Claro, SP
dc.identifierhttp://dx.doi.org/10.1186/1742-4933-10-8
dc.identifier.citationImmunity and Ageing, v. 10, n. 1, 2013.
dc.identifier.doi10.1186/1742-4933-10-8
dc.identifier.file2-s2.0-84874150350.pdf
dc.identifier.issn1742-4933
dc.identifier.scopus2-s2.0-84874150350
dc.identifier.urihttp://hdl.handle.net/11449/74628
dc.identifier.wosWOS:000327218400001
dc.language.isoeng
dc.relation.ispartofImmunity and Ageing
dc.relation.ispartofjcr4.019
dc.relation.ispartofsjr1,218
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectglucose
dc.subjectglucose 6 phosphatase
dc.subjectinsulin
dc.subjectinsulin receptor
dc.subjectinsulin receptor beta
dc.subjectinsulin receptor substrate 1
dc.subjectprotein tyrosine phosphatase 1B
dc.subjectunclassified drug
dc.subjectaging
dc.subjectanimal experiment
dc.subjectcontrolled study
dc.subjectdiet restriction
dc.subjectenzyme blood level
dc.subjectenzyme regulation
dc.subjectepididymis fat
dc.subjectexercise
dc.subjectglucose blood level
dc.subjectgroups by age
dc.subjectinsulin blood level
dc.subjectinsulin sensitivity
dc.subjectinsulin tolerance test
dc.subjectliver
dc.subjectmolecular mechanics
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectrat
dc.subjectsignal transduction
dc.subjectswimming
dc.titleAcute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old ratsen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license

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