Evaluation of DNA polymorphisms amplified by arbitrary primers (RAPD) as genetically associated elements to differentiate virulent and non-virulent Paracoccidioides brasiliensis isolates

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dc.contributor.authorMotta, Teresa R.
dc.contributor.authorMoreira-Filho, Carlos A.
dc.contributor.authorMendes, Rinaldo Poncio [UNESP]
dc.contributor.authorSouza, Lenice R. [UNESP]
dc.contributor.authorSugizak, Maria F. [UNESP]
dc.contributor.authorBaueb, Sonia
dc.contributor.authorCalich, Vera L.G.
dc.contributor.authorVaz, Celideia A.C.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:20:29Z
dc.date.available2014-05-27T11:20:29Z
dc.date.issued2002-07-12
dc.description.abstractRandomly amplified polymorphic DNA (RAPD) analysis of 35 Paracoccidioides brasiliensis isolates was carried out to evaluate the correlation of RAPD profiles with the virulence degree or the type of the clinical manifestations of human paracoccidioidomycosis. The dendrogram presented two main groups sharing 64% genetic similarity. Group A included two isolates from patients with chronic paracoccidioidomycosis; group B comprised the following isolates showing 65% similarity: two non-virulent, six attenuated, five virulent, eight from patients with chronic paracoccidioidomycosis and two from patients with acute paracoccidioidomycosis. The virulent Pb18 isolate and six attenuated or non-virulent samples derived from it were genetically indistinguishable (100% of similarity). Thus, in our study, RAPD patterns could not discriminate among 35 P. brasiliensis isolates according to their differences either in the degree of virulence or in the type of the clinical manifestation of this fungal infection. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.en
dc.description.affiliationDepartamento de Imunologia Instituto de Ciências Biomédicas USP, São Paulo, SP
dc.description.affiliationFaculdade de Medicina UNESP, Botucatu, SP
dc.description.affiliationInstituto de Biociências UNESP, Botucatu, SP
dc.description.affiliationUnespFaculdade de Medicina UNESP, Botucatu, SP
dc.description.affiliationUnespInstituto de Biociências UNESP, Botucatu, SP
dc.format.extent151-157
dc.identifierhttp://dx.doi.org/10.1016/S0928-8244(02)00306-1
dc.identifierhttp://dx.doi.org/10.1111/j.1574-695X.2002.tb00585.x
dc.identifier.citationFEMS Immunology and Medical Microbiology, v. 33, n. 3, p. 151-157, 2002.
dc.identifier.doi10.1016/S0928-8244(02)00306-1
dc.identifier.file2-s2.0-0037067639.pdf
dc.identifier.issn0928-8244
dc.identifier.scopus2-s2.0-0037067639
dc.identifier.urihttp://hdl.handle.net/11449/66933
dc.language.isoeng
dc.relation.ispartofFEMS Immunology and Medical Microbiology
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectArbitrary primers
dc.subjectDNA polymorphism
dc.subjectParacoccidioides brasiliensis
dc.subjectRandomly amplified polymorphic DNA
dc.subjectVirulent isolates
dc.subjectfungal DNA
dc.subjectprimer DNA
dc.subjectcontrolled study
dc.subjectfungal gene
dc.subjectfungus identification
dc.subjectfungus isolation
dc.subjectgene amplification
dc.subjectgene sequence
dc.subjectgenetic association
dc.subjecthuman
dc.subjectmycosis
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectrandom amplified polymorphic DNA
dc.subjectsequence homology
dc.subjectvirulence
dc.subjectAnimals
dc.subjectDNA Primers
dc.subjectDNA, Fungal
dc.subjectHumans
dc.subjectMice
dc.subjectParacoccidioides
dc.subjectParacoccidioidomycosis
dc.subjectPolymorphism, Genetic
dc.subjectRandom Amplified Polymorphic DNA Technique
dc.subjectVirulence
dc.titleEvaluation of DNA polymorphisms amplified by arbitrary primers (RAPD) as genetically associated elements to differentiate virulent and non-virulent Paracoccidioides brasiliensis isolatesen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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