Pathological and molecular analysis of Turkey coronavirus replication in captive wild Turkey embryos (broad-breasted bronze breeder)

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dc.contributor.authorGomes, Deriane E. [UNESP]
dc.contributor.authorRosa, Ana C. G. [UNESP]
dc.contributor.authorNobrega, Daniela F. [UNESP]
dc.contributor.authorGarcia, Andrea F. [UNESP]
dc.contributor.authorBrandão, Paulo E.
dc.contributor.authorCardoso, Tereza C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:26:06Z
dc.date.available2014-05-27T11:26:06Z
dc.date.issued2011-11-01
dc.description.abstractThe degree of genetic and pathologic variation exhibited by a turkey Coronavirus (TCoV) strain was investigated after nine serial passages in 25-day-old turkey embryos obtained from wild broad-breasted bronze breeders. In spite of spleen, liver, kidneys, cloacal bursa and thymus have been collected and analysed, the main histopathological changes were only documented in the intestine sections. Microscopic lesions were characterized as mild enteritis, low degree of enterocyte vacuolization and detachment of the intestinal villous after five consecutive passages and were considered absent in the last passages. Genealogic analysis based on S1 and S2 DNA sequences suggested that Brazilian isolate might be considered as originated from TCoV strains circulating in the United States, as 100% identity with TCoV-Gl strain. Although S1 S2 sequences from each passage revealed no significant point mutations, and no correlation could be speculate between S2 nucleotide changes and pathologic features in infected embryos. This is the first demonstration of wild turkey embryos as a model for TCoV isolation and propagation.en
dc.description.affiliationUNESP- São Paulo State University Laboratory of Virology DAPSA, Faculdade de Medicina Veterinária, Araçatuba, SP
dc.description.affiliationCoronavirus Research Group Faculdade de Medicina Veterinária e Zootecnia USP - University of São Paulo, SP
dc.description.affiliationUnespUNESP- São Paulo State University Laboratory of Virology DAPSA, Faculdade de Medicina Veterinária, Araçatuba, SP
dc.format.extent184-189
dc.identifierhttp://www.abpv.vet.br/paginas_site/default.asp?P=BJVP&PAG_SEQ=7601
dc.identifier.citationBrazilian Journal of Veterinary Pathology, v. 4, n. 3, p. 184-189, 2011.
dc.identifier.issn1983-0246
dc.identifier.scopus2-s2.0-82755186193
dc.identifier.urihttp://hdl.handle.net/11449/72780
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Veterinary Pathology
dc.relation.ispartofsjr0,162
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectPathology
dc.subjectSpike gene
dc.subjectTurkey coronavirus
dc.subjectWild turkey species
dc.titlePathological and molecular analysis of Turkey coronavirus replication in captive wild Turkey embryos (broad-breasted bronze breeder)en
dc.typeArtigo
dcterms.licensehttp://www.abpv.vet.br/paginas_site/default.asp?P=BJVP&PAG_SEQ=4282
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

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