Testicular morphological changes during the seasonal reproductive cycle in the bullfrog Rana catesbeiana

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dc.contributor.authorCerri, Estela Sasso [UNESP]
dc.contributor.authorDe Faria, Flávio P.
dc.contributor.authorFreymüller, Edna
dc.contributor.authorMiraglia, Sandra M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:21:02Z
dc.date.available2014-05-27T11:21:02Z
dc.date.issued2004-03-01
dc.description.abstractSpermatogenesis and steroidogenesis undergo seasonal variations during the reproductive cycle in amphibians. Testicular morphological and morphometric seasonal variations as well as interstitial lipidic inclusions and intralobular glycoconjugates were evaluated during seasonal cycle of Rana catesbeiana. Testes of frogs collected during the annual seasons were weighed for calculation of GSI (Gonadosomatic index). Seminiferous lobule diameters (DSL) and volume densities of seminiferous lobules (VvSL), excretory ducts (VvED), and interstitial tissue (VvIT) were analyzed. Semithin sections were submitted to Periodic Acid-Schiff (PAS) and Alcian Blue (AB) methods for detection of glycoconjugates, while lipidic inclusions were detected by Sudan Black B. GSI showed no significant variations during the year. Since VvED and VvIT increased significantly during summer and were inversely proportional to VvSL, a compensatory effect between the testicular compartments may be related to the maintenance of GSI. During autumn/winter, larger lobular diameters were observed in comparison to spring/summer when spermiogenesis and spermiation were commonly observed. The increased VvIT and the numerous lipidic inclusions in the interstitial cells during summer suggest a relationship between spermiogenesis and steroidogenesis. Besides the structural stability variations occurring in the IT and SL, a possible paracrine interaction between ED and IT should be also involved in the IT development during summer. The presence of PAS and AB-positive globular structures were observed in the seminiferous lobules and excretory ducts. These structures containing acid glycoconjugates appear to be Sertoli cell apical portions, which are accumulated in the lumen of the seminiferous lobules mainly during spermiation. © 2004 Wiley-Liss, Inc.en
dc.description.affiliationDepartment of Morphology São Paulo State University, Rua Humaitá, 1680, Araraquara (SP)
dc.description.affiliationElectron Microscopy Center Fed. University of São Paulo, Rua Botucatu, 862, Sao Paulo (SP)
dc.description.affiliationDepartment of Morphology Fed. University of São Paulo, Rua Botucatu, 740, Sao Paulo (SP)
dc.description.affiliationUnespDepartment of Morphology São Paulo State University, Rua Humaitá, 1680, Araraquara (SP)
dc.format.extent249-260
dc.identifierhttp://dx.doi.org/10.1002/jez.a.20023
dc.identifier.citationJournal of Experimental Zoology Part A: Comparative Experimental Biology, v. 301, n. 3, p. 249-260, 2004.
dc.identifier.doi10.1002/jez.a.20023
dc.identifier.issn0022-104X
dc.identifier.lattes4455630076841302
dc.identifier.scopus2-s2.0-1542684105
dc.identifier.urihttp://hdl.handle.net/11449/67663
dc.language.isoeng
dc.relation.ispartofJournal of Experimental Zoology Part A: Comparative Experimental Biology
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectalcian blue
dc.subjectazo compound
dc.subjectglycoconjugate
dc.subjectsudan black
dc.subjectanalysis of variance
dc.subjectanimal
dc.subjectcomparative study
dc.subjectfrog
dc.subjecthistology
dc.subjectmale
dc.subjectmetabolism
dc.subjectmorphometrics
dc.subjectperiodic acid Schiff stain
dc.subjectphysiology
dc.subjectreproduction
dc.subjectseason
dc.subjecttestis
dc.subjectAlcian Blue
dc.subjectAnalysis of Variance
dc.subjectAnimals
dc.subjectAzo Compounds
dc.subjectBody Weights and Measures
dc.subjectGlycoconjugates
dc.subjectHistological Techniques
dc.subjectMale
dc.subjectPeriodic Acid-Schiff Reaction
dc.subjectRana catesbeiana
dc.subjectReproduction
dc.subjectSeasons
dc.subjectTestis
dc.subjectAmphibia
dc.subjectAnura
dc.titleTesticular morphological changes during the seasonal reproductive cycle in the bullfrog Rana catesbeianaen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.author.lattes4455630076841302
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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