Use of near-infrared Raman spectroscopy to detect IgG and IgM antibodies against Toxoplasma gondii in serum samples of domestic cats

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dc.contributor.authorDuarte, J.
dc.contributor.authorPacheco, MTT
dc.contributor.authorMachado, R. Z.
dc.contributor.authorSilveira, L.
dc.contributor.authorZangaro, R. A.
dc.contributor.authorVillaverde, A. B.
dc.contributor.institutionUniv Vale Paraiba
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:15:50Z
dc.date.available2014-05-20T13:15:50Z
dc.date.issued2002-07-01
dc.description.abstractNear-infrared Raman spectroscopy (NIRS) is a particularly promising technique that is being used in recent years for many biomedical applications. Optical spectroscopy has gained increasing prominence as a tool for quantitative analysis of biological samples, clinical diagnostic, concentration measurements of blood metabolites and therapeutic drugs, and analysis of the chemical composition of human tissues. Toxoplasmosis is an important zoonosis in public health, and domestic cats are the most important transmitters of the disease. This disease can be detected by several serological tests, which usually have a high cost and require a long time. The goal of this work was to investigate a new method to diagnosis Toxoplasma gondii infections using NIRS. In order to confirm antibody detection, 24 cat blood scrum samples were analyzed by the Raman spectra, from which 23 presented positive serology to toxoplasmosis and one was a reference negative serum. Characteristic Raman peaks allowed differentiation between negative and positive sera, confirming the possibility of antibody detection by Raman spectroscopy. These results give the first evidence that this technique can be useful to quantify antibodies in cat sera.en
dc.description.affiliationUniv Vale Paraiba, Inst Pesquisa & Desenvolvimento, BR-12244000 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Patol Vet, BR-14870000 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Patol Vet, BR-14870000 Jaboticabal, SP, Brazil
dc.format.extent585-589
dc.identifier.citationCellular and Molecular Biology. Noisy-le-grand: Cellular & Molecular Biology, v. 48, n. 5, p. 585-589, 2002.
dc.identifier.issn0145-5680
dc.identifier.lattes3254990612451836
dc.identifier.urihttp://hdl.handle.net/11449/2872
dc.identifier.wosWOS:000176840900024
dc.language.isoeng
dc.publisherCellular & Molecular Biology
dc.relation.ispartofCellular and Molecular Biology
dc.relation.ispartofjcr1.372
dc.relation.ispartofsjr0,457
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectimmunoglobulinpt
dc.subjecttoxoplasmosispt
dc.subjectRaman spectroscopypt
dc.subjectCatpt
dc.subjectnear-infrared spectroscopypt
dc.titleUse of near-infrared Raman spectroscopy to detect IgG and IgM antibodies against Toxoplasma gondii in serum samples of domestic catsen
dc.typeArtigo
dcterms.rightsHolderCellular & Molecular Biology
unesp.author.lattes3254990612451836
unesp.author.orcid0000-0002-6616-3334[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Patologia Veterinária - FCAV