Blood cells attachment after root conditioning and PRP application: An in vitro study

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dc.contributor.authorDantas, Andréa Abi Rached [UNESP]
dc.contributor.authorFontanari, Lucas Amaral [UNESP]
dc.contributor.authorde Paula Ishi, Eduardo
dc.contributor.authorLeite, Fábio Renato Manzolli
dc.contributor.authorZandim-Barcelos, Daniela Leal [UNESP]
dc.contributor.authorRached, Ricardo Samih Georges Abi [UNESP]
dc.contributor.authorSampaio, José Eduardo Cezar [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionSão Paulo
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.date.accessioned2014-05-27T11:26:29Z
dc.date.available2014-05-27T11:26:29Z
dc.date.issued2012-05-01
dc.description.abstractAim: Root conditioning is aimed at smear layer removal and at dental matrix collagen exposure, which may promote periodontal regeneration. This in vitro study assessed smear layer removal, collagen fiber exposure and the influence of PRP (platelet-rich plasma) application on adhesion of blood cells to the root surface using scanning electron microscopy (SEM). Materials and methods: Scaled root samples (n = 160) were set in five groups and conditioned with: group I - control group (saline solution); group II (EDTA 24%); group III (citric acid 25%); group IV (tetracycline hydrochloride 50 mg/ml); group V (sodium citrate 30%). Eighty samples were assessed using the root surface modification index (RSMI). The other eighty samples were set in two groups. The first group (n = 40) received PRP gel application with a soft brush and the second group (n = 40) received PRP application and then a blood drop. The fibrin clot formation was assessed in the first group and the blood cells adhesion was assessed in the second group using the BEAI (blood elements adhesion index). A previously trained, calibrated, and blind examiner evaluated photomicrographs. Statistical analysis was performed using the Kruskal-Wallis's and Dunn's tests. Results: Group III attained the best results for RSMI and BEAI. Moreover, it was the only group showing fibrin clot formation. Conclusion: Citric acid was the most efficient conditioner for smear layer removal, collagen fiber exposure and blood cell adhesion. Moreover, it was the only group showing fibrin clot formation after PRP application. Clinical significance: This study demonstrated that root conditioning followed by PRP application may favor blood cell adhesion on root surface which may optimize periodontal healing.en
dc.description.affiliationDepartment of Operative Dentistry School of Dentistry at Araraquara State University of São Paulo, UNESP, 1680 Humaita Street, Araraquara
dc.description.affiliationDepartment of Diagnostic and Surgery School of Dentistry at Araraquara State University of São Paulo, UNESP, São Paulo
dc.description.affiliationSão Paulo
dc.description.affiliationDepartment of Semiology and Clinics School of Dentistry of Pelotas Federal University of Pelotas, UFPel, Rio Grande do Sul
dc.description.affiliationUnespDepartment of Operative Dentistry School of Dentistry at Araraquara State University of São Paulo, UNESP, 1680 Humaita Street, Araraquara
dc.description.affiliationUnespDepartment of Diagnostic and Surgery School of Dentistry at Araraquara State University of São Paulo, UNESP, São Paulo
dc.format.extent332-338
dc.identifierhttp://dx.doi.org/10.5005/jp-journals-10024-1147
dc.identifier.citationJournal of Contemporary Dental Practice, v. 13, n. 3, p. 332-338, 2012.
dc.identifier.doi10.5005/jp-journals-10024-1147
dc.identifier.file2-s2.0-84865278653.pdf
dc.identifier.issn1526-3711
dc.identifier.lattes9531839078426485
dc.identifier.scopus2-s2.0-84865278653
dc.identifier.urihttp://hdl.handle.net/11449/73311
dc.language.isoeng
dc.relation.ispartofJournal of Contemporary Dental Practice
dc.relation.ispartofsjr0,263
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBlood
dc.subjectCitric acid
dc.subjectEdetic acid
dc.subjectLaboratory research
dc.subjectPlatelet-rich plasma
dc.subjectRoot conditioning
dc.subjectSmear layer
dc.subjectTetracycline
dc.subjectchelating agent
dc.subjectcitrate sodium
dc.subjectcitric acid
dc.subjectedetic acid
dc.subjectfibrin
dc.subjecttetracycline
dc.subjectblood cell
dc.subjectblood clotting
dc.subjectcell adhesion
dc.subjectcementum
dc.subjectdental surgery
dc.subjectdentin
dc.subjectdrug effect
dc.subjecthuman
dc.subjectmale
dc.subjectphysiology
dc.subjectreparative dentistry
dc.subjectscanning electron microscopy
dc.subjectthrombocyte rich plasma
dc.subjecttooth disease
dc.subjecttooth root
dc.subjectultrastructure
dc.subjectBlood Cells
dc.subjectBlood Coagulation
dc.subjectCell Adhesion
dc.subjectChelating Agents
dc.subjectCitrates
dc.subjectCitric Acid
dc.subjectDental Cementum
dc.subjectDentin
dc.subjectEdetic Acid
dc.subjectFibrin
dc.subjectHumans
dc.subjectMale
dc.subjectMicroscopy, Electron, Scanning
dc.subjectPlatelet-Rich Plasma
dc.subjectSmear Layer
dc.subjectTissue Conditioning (Dental)
dc.subjectTooth Demineralization
dc.subjectTooth Root
dc.titleBlood cells attachment after root conditioning and PRP application: An in vitro studyen
dc.typeArtigo
dcterms.licensehttp://www.thejcdp.com/pdfs/copyright_transfer_form.pdf
unesp.author.lattes9531839078426485
unesp.author.lattes7460825090327014[1]
unesp.author.orcid0000-0002-8309-8837[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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