Safety assessment of oral photodynamic therapy in rats

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dc.contributor.authorFontana, Carla R. [UNESP]
dc.contributor.authorLerman, Mark A.
dc.contributor.authorPatel, Niraj
dc.contributor.authorGrecco, Clovis
dc.contributor.authorSouza Costa, Carlos A. de [UNESP]
dc.contributor.authorAmiji, Mansoor M.
dc.contributor.authorBagnato, Vanderlei S.
dc.contributor.authorSoukos, Nikolaos S.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionHarvard Univ
dc.contributor.institutionForsyth Inst
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionNortheastern University
dc.date.accessioned2014-12-03T13:11:42Z
dc.date.available2014-12-03T13:11:42Z
dc.date.issued2013-02-01
dc.description.abstractPhotodynamic therapy (PDT) is based on the synergism of a photosensitive drug (a photosensitizer) and visible light to destroy target cells (e.g., malignant, premalignant, or bacterial cells). The aim of this study was to investigate the response of normal rat tongue mucosa to PDT following the topical application of hematoporphyrin derivative (PhotogemA (R)), PhotodithazineA (R), methylene blue (MB), and poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with MB. One hundred and thirty three rats were randomly divided in various groups: the PDT groups were treated with the photosensitizers for 10 min followed by exposure to red light. Those in control groups received neither photosensitizer nor light, and they were subjected to light exposure alone or to photosensitizer alone. Fluorescent signals were obtained from tongue tissue immediately after the topical application of photosensitizers and 24 h following PDT. Histological changes were evaluated at baseline and at 1, 3, 7, and 15 days post-PDT treatment. Fluorescence was detected immediately after the application of the photosensitizers, but not 24 h following PDT. Histology revealed intact mucosa in all experimental groups at all evaluation time points. The results suggest that there is a therapeutic window where PDT with PhotogemA (R), PhotodithazineA (R), MB, and MB-loaded PLGA nanoparticles could safely target oral pathogenic bacteria without damaging normal oral tissue.en
dc.description.affiliationUniv Sao Paulo State UNESP, Sch Pharmaceut Sci, Dept Clin Anal, BR-14801960 Araraquara, Sau Paulo, Brazil
dc.description.affiliationHarvard Univ, Sch Dent Med, Dept Oral Med Infect & Immun, Boston, MA 02115 USA
dc.description.affiliationForsyth Inst, Appl Mol Photomed Lab, Boston, MA 02115 USA
dc.description.affiliationUniv Sao Paulo, Inst Phys Sao Carlos, BR-15980900 Sao Carlos, Sau Paulo, Brazil
dc.description.affiliationUniv Sao Paulo State UNESP, Dent Sch Araraquara, BR-14801960 Araraquara, Sau Paulo, Brazil
dc.description.affiliationNortheastern Univ, Bouve Coll Hlth Sci, Sch Pharm, Dept Pharmaceut Sci, Boston, MA 02115 USA
dc.description.affiliationUnespUniv Sao Paulo State UNESP, Sch Pharmaceut Sci, Dept Clin Anal, BR-14801960 Araraquara, Sau Paulo, Brazil
dc.description.affiliationUnespUniv Sao Paulo State UNESP, Dent Sch Araraquara, BR-14801960 Araraquara, Sau Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent479-486
dc.identifierhttp://dx.doi.org/10.1007/s10103-012-1091-6
dc.identifier.citationLasers In Medical Science. London: Springer London Ltd, v. 28, n. 2, p. 479-486, 2013.
dc.identifier.doi10.1007/s10103-012-1091-6
dc.identifier.issn0268-8921
dc.identifier.urihttp://hdl.handle.net/11449/113435
dc.identifier.wosWOS:000315579400018
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofLasers in Medical Science
dc.relation.ispartofjcr1.949
dc.relation.ispartofsjr0,713
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPhotodynamic therapyen
dc.subjectPhotosensitizersen
dc.subjectHistological analysisen
dc.subjectFluorescenceen
dc.titleSafety assessment of oral photodynamic therapy in ratsen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes5168319315634298[1]
unesp.author.lattes4517484241515548[5]
unesp.author.orcid0000-0002-9135-3690[1]
unesp.author.orcid0000-0002-7455-6867[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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