Release of intermediate reactive hydrogen peroxide by macrophage cells activated by natural products

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dc.contributor.authorMoreira, RRD
dc.contributor.authorCarlos, I. Z.
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:24:03Z
dc.date.available2014-05-20T15:24:03Z
dc.date.issued2001-02-01
dc.description.abstractBy determining the hydrogen peroxide (H2O2) released in cultures of peritoneal macrophage cells from Swiss mice, we evaluated the action of 27 vegetable compounds (pristimerin, tingenone, jatrophone, palustric acid, lupeol, cladrastin, ocoteine, boldine, tomatine, yohimbine, reserpine, escopoletin, esculine, plumericin, diosgenin, deoxyschizandrin, p-arbutin, mangiferin, and others) using a 2 mg/ml solution of each compound (100 mug/well). Macrophages are cells responsible for the development of the immunological response reaction, liberating more than one hundred compounds into the extracellular environment. Among these are the various cytokines and the intermediate compounds of nitrogen (NO) and oxygen (H2O2). This coordinated sequence of biochemical reactions is known as the oxidative burst. When we compared the results with those obtained with zymosan (an important stimulator of H2O2) we observed that the compounds showing the highest activity were substances 2 (tingenone), 16 (reserpine) and 20. Other substances such as compounds 1, 4, 5, 6, 8, 12, 13, 14, 15, 17, 19, 23, 24, 26, and 27 also showed a certain activity, but with less intensity than the aforementioned ones. Compounds 3, 7, 9, 10, 11, 18, 21, 22 and 25 presented no activity. These results suggest that natural products (mainly tingenone and reserpine and others) with different chemical structures are strong immunological modulators. However, further tests are needed to determine the 'oxidative burst' in future studies.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Farmaceut, Dept Principios Ativos Nat & Toxicol, BR-14801902 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Farmaceut, Dept Anal Clin, BR-14801902 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Dept Quim Organ, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Farmaceut, Dept Principios Ativos Nat & Toxicol, BR-14801902 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Farmaceut, Dept Anal Clin, BR-14801902 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Dept Quim Organ, São Paulo, Brazil
dc.format.extent201-204
dc.identifierhttp://dx.doi.org/10.1248/bpb.24.201
dc.identifier.citationBiological & Pharmaceutical Bulletin. Tokyo: Pharmaceutical Soc Japan, v. 24, n. 2, p. 201-204, 2001.
dc.identifier.doi10.1248/bpb.24.201
dc.identifier.fileWOS000166743100020.pdf
dc.identifier.issn0918-6158
dc.identifier.orcid0000-0003-3032-2556
dc.identifier.urihttp://hdl.handle.net/11449/34706
dc.identifier.wosWOS:000166743100020
dc.language.isoeng
dc.publisherPharmaceutical Soc Japan
dc.relation.ispartofBiological & Pharmaceutical Bulletin
dc.relation.ispartofjcr1.694
dc.relation.ispartofsjr0,626
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectnatural productpt
dc.subjectmacrophagept
dc.subjecthydrogen peroxidept
dc.titleRelease of intermediate reactive hydrogen peroxide by macrophage cells activated by natural productsen
dc.typeArtigo
dcterms.licensehttp://bpb.pharm.or.jp/document/transfer.pdf
dcterms.rightsHolderPharmaceutical Soc Japan
unesp.author.orcid0000-0003-3032-2556[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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