Chromosomal diversification of diploid number, heterochromatin and rDNAs in two species of Phanaeus beetles (Scarabaeidae, Scarabaeinae)

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dc.contributor.authorArcanjo, Amanda
dc.contributor.authorCabral-de-Mello, Diogo Cavalcanti [UNESP]
dc.contributor.authorMartins, Cesar [UNESP]
dc.contributor.authorMoura, Rita de Cássia de
dc.contributor.authorSouza, Maria José de
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade de Pernambuco
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:40Z
dc.date.available2014-05-27T11:30:40Z
dc.date.issued2013-09-05
dc.description.abstractThe genus Phanaeus is included in the tribe Phanaeini, one of the most diverse tribes within the subfamily Scarabaeinae in terms of chromosomal characteristics. However, so far the species of this genus were not studied with differential cytogenetic techniques, limiting any inference of the probable mechanisms responsible for this diversity. In this work, several techniques were applied with the aim of cytogenetically characterizing two Phanaeus species. The karyotype found for Phanaeus (Notiophanaeus) chalcomelas was 2n = 12, neo-XY, and that of P. (N.) splendidulus was 2n = 20, Xyp, considered primitive for the family Scarabaeidae. The chromosomes of both species showed a high amount of constitutive heterochromatin (CH), with blocks rich in base pairs GC (CMA3 +). Moreover, in P. (N.) chalcomelas the marks revealed by C-banding and fluorochrome staining were different in size, showing CH variability. Sites of 18S ribosomal DNA (rDNA) were identified in one autosomal pair of P. (N.) chalcomelas and in five autosomal pairs of P. (N.) splendidulus. On the other hand, only one autosomal pair exhibited 5S rDNA sequences in these species. The results suggest that the karyotype differentiation of the Phanaeus species studied here involved pericentric inversions and centric fusions, as well as mechanisms related to amplification and dispersion of CH and rDNA sequences. © 2013, Sociedade Brasileira de Genética.en
dc.description.affiliationDepartamento de Genética Centro de Ciências Biológicas Universidade Federal de Pernambuco, Recife, PE
dc.description.affiliationDepartamento de Biologia Instituto de Ciências Biológicas Universidade de Pernambuco, Recife, PE
dc.description.affiliationDepartamento de Biologia Instituto de Biociências Universidade Estadual Paulista 'Júlio de Mesquita Filho', Rio Claro, SP
dc.description.affiliationDepartamento de Morfologia Instituto de Biociências Universidade Estadual Paulista 'Júlio de Mesquita Filho', Botucatu, SP
dc.description.affiliationUnespDepartamento de Biologia Instituto de Biociências Universidade Estadual Paulista 'Júlio de Mesquita Filho', Rio Claro, SP
dc.description.affiliationUnespDepartamento de Morfologia Instituto de Biociências Universidade Estadual Paulista 'Júlio de Mesquita Filho', Botucatu, SP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundacao de Amparo a Ciência e Tecnologia do Estado de Pernambuco
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFACEPE - BPD-003.2.02/08 e APQ-0464-2.02/10
dc.format.extent341-346
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572013005000031
dc.identifier.citationGenetics and Molecular Biology, v. 36, n. 3, p. 341-346, 2013.
dc.identifier.doi10.1590/S1415-47572013005000031
dc.identifier.file2-s2.0-84883275469.pdf
dc.identifier.issn1415-4757
dc.identifier.issn1678-4685
dc.identifier.lattes8858800699425352
dc.identifier.orcid0000-0003-3534-974X
dc.identifier.scieloS1415-47572013005000031
dc.identifier.scopus2-s2.0-84883275469
dc.identifier.urihttp://hdl.handle.net/11449/76529
dc.identifier.wosWOS:000323725500007
dc.language.isoeng
dc.relation.ispartofGenetics and Molecular Biology
dc.relation.ispartofjcr1.493
dc.relation.ispartofsjr0,638
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectChromosome evolution
dc.subjectFISH
dc.subjectKaryotype
dc.subjectRepetitive DNA
dc.subjectDNA 18S
dc.subjectDNA 5S
dc.subjectribosome DNA
dc.subjectanimal tissue
dc.subjectautosome
dc.subjectbase pairing
dc.subjectbeetle
dc.subjectC banding
dc.subjectchromosomal diversification
dc.subjectchromosome NOR
dc.subjectchromosomes and chromosomal phenomena
dc.subjectdiploidy
dc.subjectDNA sequence
dc.subjectfluorescence in situ hybridization
dc.subjectfluorochrome staining
dc.subjectgene mapping
dc.subjectheterochromatin
dc.subjectkaryotype
dc.subjectmale
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpericentric chromosome inversion
dc.subjectPhanaeus
dc.subjectPhanaeus (Notiophanaeus) chalcomelas
dc.subjectPhanaeus (Notiophanaeus) splendidulus
dc.titleChromosomal diversification of diploid number, heterochromatin and rDNAs in two species of Phanaeus beetles (Scarabaeidae, Scarabaeinae)en
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/gmb/iaboutj.htm
unesp.author.lattes8858800699425352[3]
unesp.author.orcid0000-0003-3534-974X[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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