Paradoxical effects of bovine somatotropin treatment on the ovarian follicular population and in vitro embryo production of lactating buffalo donors submitted to ovum pick-up

Página do item simplificado
dc.contributor.authorFerraz, Márcio Leão
dc.contributor.authorSa Filho, Manoel Francisco de
dc.contributor.authorBatista, Emiliana Oliveira Santana
dc.contributor.authorWatanabe, Yeda Fumie
dc.contributor.authorWatanabe, Michele Rodrigues
dc.contributor.authorDayan, André
dc.contributor.authorJoaquim, Daniel Carlino
dc.contributor.authorAccorsi, Monica R.
dc.contributor.authorGimenes, Lindsay Unno [UNESP]
dc.contributor.authorVieira, Laís Mendes
dc.contributor.authorBaruselli, Pietro Sampaio
dc.contributor.institutionVida Reprod Consultoria SS LTDA
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionVitrogen
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-10-21T20:11:40Z
dc.date.available2015-10-21T20:11:40Z
dc.date.issued2015-03-01
dc.description.abstractThe aim of the present study was to evaluate the effect of bovine somatotropin (bST; 500 mg) administration on lactating buffalo donors submitted to two different ovum pick-up (OPU) and in vitro embryo production schemes with a 7 or 14 d intersession OPU interval. A total of 16 lactating buffalo cows were randomly assigned into one of four experimental groups according to the bST treatment (bST or No-bST) and the OPU intersession interval (7 or 14 d) in a 2 x 2 factorial design (16 weeks of OPU sessions). The females submitted to OPU every 14d had a larger (P < 0.001) number of ovarian follicles suitable for puncture (15.6 +/- 0.7 vs. 12.8 +/- 0.4) and an increased (P = 0.004) number of cumulus-oocyte complexes (COCs) recovered (10.0 +/- 0.5 vs. 8.5 +/- 0.3) compared to the 7 d interval group. However, a 7 or 14 d interval between OPU sessions had no effect (P = 0.34) on the number of blastocysts produced per OPU (1.0 +/- 0.1 vs. 13 +/- 0.2, respectively). In addition, bST treatment increased (P < 0.001) the number of ovarian follicles suitable for puncture (15.3 +/- 0.5 vs. 12.1 +/- 0.4) but reduced the percentage (18.9% vs. 10.9%; P = 0.009) and the number (1.4 +/- 0.2 vs. 0.8 +/- 0.1; P = 0.003) of blastocysts produced per OPU session compared with the non-bST-treated buffaloes. In conclusion, the 14d interval between OPU sessions and bST treatment efficiently increased the number of ovarian follicles suitable for puncture. However, the OPU session interval had no effect on embryo production, and bST treatment reduced the in vitro blastocyst outcomes in lactating buffalo donors.en
dc.description.affiliationUniversidade de São Paulo, Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal
dc.description.sponsorshipVida Reprodutiva Consultoria - Serviços em Reprodução Animal
dc.description.sponsorshipVitrogen
dc.format.extent1-7
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0378432015000020
dc.identifier.citationAnimal Reproduction Science. Amsterdam: Elsevier Science Bv, v. 154, p. 1-7, 2015.
dc.identifier.doi10.1016/j.anireprosci.2014.12.017
dc.identifier.issn0378-4320
dc.identifier.lattes9472065929531215
dc.identifier.urihttp://hdl.handle.net/11449/129007
dc.identifier.wosWOS:000350927500001
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofAnimal Reproduction Science
dc.relation.ispartofjcr1.647
dc.relation.ispartofsjr0,704
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectOPUen
dc.subjectbSTen
dc.subjectEmbryoen
dc.subjectIGFen
dc.subjectOocyte qualityen
dc.titleParadoxical effects of bovine somatotropin treatment on the ovarian follicular population and in vitro embryo production of lactating buffalo donors submitted to ovum pick-upen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes9472065929531215
unesp.author.orcid0000-0002-7644-675X[9]
unesp.author.orcid0000-0002-6773-4450[11]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

Arquivos

Coleções

Artigos - Medicina Veterinária Preventiva e Reprodução Animal - FCAV