The effects of male age on sperm DNA damage in an infertile population

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dc.contributor.authorVagnini, Laura
dc.contributor.authorBaruffi, R. L R
dc.contributor.authorMauri, A. L. [UNESP]
dc.contributor.authorPetersen, C. G. [UNESP]
dc.contributor.authorMassaro, F. C.
dc.contributor.authorPontes, Anaglória [UNESP]
dc.contributor.authorOliveira, J. B A
dc.contributor.authorFranco, J. G. [UNESP]
dc.contributor.institutionCPDP-Paulista Centre for Diagnosis and Research
dc.contributor.institutionCentre for Human Reproduction Prof. Franco Junior
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:22:37Z
dc.date.available2014-05-27T11:22:37Z
dc.date.issued2007-11-01
dc.description.abstractThe objective was to investigate the influence of age on sperm DNA damage. Semen samples were collected from 508 men in an unselected group of couples attending infertility investigation and treatment. DNA fragmentation in spermatozoa was measured by TdT (terminal deoxynucleotidyl transferase)-mediated dUTP nick-end labelling (TUNEL) assay; at least 200 spermatozoa in randomly selected areas of microscope slides were evaluated using a fluorescent microscope and the percentage of TUNEL positive spermatozoa was determined. The number of cells with red fluorescence (TUNEL positive) was expressed as a percentage of the total sample [DNA fragmentation index (DFI)]. Age was treated as a continuous variable for regression and correlation analysis. The following male age groups were used: Group I: ≤35 years, Group II: 36-39 years, and Group III: ≥40 years. DFI was significantly lower in Group I than in Group II (P = 0.034) or III (P = 0.022). There was no difference in DFI between Groups II and III. In addition, regression analysis demonstrated a significant increase in sperm DFI with age (P = 0.02). TUNEL assay clearly demonstrates an increase in sperm DNA damage with age. © 2007 Published by Reproductive Healthcare Ltd.en
dc.description.affiliationCPDP-Paulista Centre for Diagnosis and Research, Ribeirão Preto, SP
dc.description.affiliationCentre for Human Reproduction Prof. Franco Junior, Av. Prof. João Fiusa, 14025-310 Ribeirão Preto, SP
dc.description.affiliationDepartment of Gynaecology and Obstetrics Botucatu School of Medicine UNESP, Botucatu, SP
dc.description.affiliationDepartment of Gynaecology and Obstetrics Botucatu School of Medicine (UNESP), Botucatu, SP
dc.description.affiliationUnespDepartment of Gynaecology and Obstetrics Botucatu School of Medicine UNESP, Botucatu, SP
dc.description.affiliationUnespDepartment of Gynaecology and Obstetrics Botucatu School of Medicine (UNESP), Botucatu, SP
dc.format.extent514-519
dc.identifierhttp://dx.doi.org/10.1016/S1472-6483(10)60382-3
dc.identifier.citationReproductive BioMedicine Online, v. 15, n. 5, p. 514-519, 2007.
dc.identifier.doi10.1016/S1472-6483(10)60382-3
dc.identifier.issn1472-6483
dc.identifier.lattes0514178654667684
dc.identifier.scopus2-s2.0-36448967516
dc.identifier.urihttp://hdl.handle.net/11449/69947
dc.identifier.wosWOS:000250886600005
dc.language.isoeng
dc.relation.ispartofReproductive BioMedicine Online
dc.relation.ispartofjcr2.967
dc.relation.ispartofsjr1,343
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAge
dc.subjectDNA damage
dc.subjectMale infertility
dc.subjectSpermatozoa
dc.subjectTUNEL
dc.subjectDNA
dc.subjectDNA fragment
dc.subjectadult
dc.subjectage
dc.subjectasthenospermia
dc.subjectcontrolled study
dc.subjectcorrelation analysis
dc.subjectfluorescence microscopy
dc.subjecthuman
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmale infertility
dc.subjectnick end labeling
dc.subjectoligospermia
dc.subjectregression analysis
dc.subjectsemen analysis
dc.subjectspermatozoon
dc.subjectAdult
dc.subjectAged
dc.subjectAging
dc.subjectDNA Damage
dc.subjectHumans
dc.subjectIn Situ Nick-End Labeling
dc.subjectInfertility, Male
dc.subjectMale
dc.subjectMiddle Aged
dc.titleThe effects of male age on sperm DNA damage in an infertile populationen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes0514178654667684
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Ginecologia e Obstetrícia - FMB