Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

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dc.contributor.authorMiyazaki, Celina Massumi
dc.contributor.authorPereira, Tamyris Paschoal
dc.contributor.authorMascagni, Daniela Branco Tavares [UNESP]
dc.contributor.authorMoraes, Marli Leite de
dc.contributor.authorFerreira, Marystela
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-12-07T15:40:49Z
dc.date.available2015-12-07T15:40:49Z
dc.date.issued2016-01-01
dc.description.abstractIn this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. The LbL film growth was monitored by surface plasmon resonance (SPR) by gold resonance angle shift analysis after each bilayer deposition. Subsequently, the films were applied as amperometric biosensors for dopamine detection using Prussian Blue (PB) as the electron mediator. The biosensor fabricated by MAO-B incorporated into liposomes composed of DPPG:POPG in the ratio (1:4) (w/w) showed the best performance with a sensitivity of 0.86 (μA cm− 2)/(mmol L− 1) and a detection limit of 0.33 mmol L− 1.en
dc.description.affiliationUniversidade Federal de São Carlos, UFSCar, CCTS, Sorocaba, São Paulo, Brazil.
dc.description.affiliationUniversidade Estadual de São Paulo - UNESP, Sorocaba, São Paulo, Brazil.
dc.description.affiliationUniversidade Federal de São Paulo, Unifesp, São José dos Campos, São Paulo, Brazil.
dc.description.affiliationUniversidade Federal de São Carlos, UFSCar, CCTS, Sorocaba, São Paulo, Brazil. Electronic address: marystela@ufscar.br.
dc.description.affiliationUnespUniversidade Estadual de São Paulo - UNESP, Sorocaba, São Paulo, Brazil.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2014/15093-7
dc.description.sponsorshipIdFAPESP: 2014/17519-1
dc.format.extent310-315
dc.identifierhttp://dx.doi.org/10.1016/j.msec.2015.08.042
dc.identifier.citationMaterials Science & Engineering. C, Materials For Biological Applications, v. 58, p. 310-315, 2016.
dc.identifier.doi10.1016/j.msec.2015.08.042
dc.identifier.issn1873-0191
dc.identifier.pubmed26478315
dc.identifier.urihttp://hdl.handle.net/11449/131697
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofMaterials Science & Engineering. C, Materials For Biological Applications
dc.relation.ispartofsjr1,110
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectAmperometryen
dc.subjectDopamine detectionen
dc.subjectLayer-by-layeren
dc.subjectLiposomesen
dc.subjectMonoamine oxidase-ben
dc.titleMonoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detectionen
dc.typeArtigo
dcterms.rightsHolderElsevier B. V.

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