COX24 codes for a mitochondrial protein required for processing of the COX1 transcript

Página do item simplificado
dc.contributor.authorBarros, Mario H. [UNESP]
dc.contributor.authorMyers, Alan M.
dc.contributor.authorVan Driesche, Sarah
dc.contributor.authorTzagoloff, Alexander
dc.contributor.institutionColumbia University
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionIowa State University
dc.date.accessioned2014-05-27T11:21:49Z
dc.date.available2014-05-27T11:21:49Z
dc.date.issued2006-02-10
dc.description.abstractIn most strains of Saccharomyces cerevisiae the mitochondrial gene COX1, for subunit 1 of cytochrome oxidase, contains multiple exons and introns. Processing of COX1 primary transcript requires accessory proteins factors, some of which are encoded by nuclear genes and others by reading frames residing in some of the introns of the COX1 and COB genes. Here we show that the low molecular weight protein product of open reading frame YLR204W, for which we propose the name COX24, is also involved in processing of COX1 RNA intermediates. The growth defect of cox24 mutants is partially rescued in strains harboring mitochondrial DNA lacking introns. Northern blot analyses of mitochondrial transcripts indicate cox24 null mutants to be blocked in processing of introns aI2 and aI3. The dependence of intron aI3 excision on Cox24p is also supported by the growth properties of the cox24 mutant harboring mitochondrial DNA with different intron compositions. The intermediate phenotype of the cox24 mutant in the background of intronless mitochondrial DNA, however, suggests that in addition to its role in splicing of the COX1 pre-mRNA, Cox24p still has another function. Based on the analysis of a cox14-cox24 double mutant, we propose that the other function of Cox24p is related to translation of the COX1 mRNA. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.en
dc.description.affiliationDepartment of Biological Sciences Columbia University, New York, NY 10027
dc.description.affiliationDept. of Genetics IBB-UNESP, Botucatu/SP 18607-741
dc.description.affiliationDept. of Biochemistry, Biophysics and Molecular Biology Iowa State University, Ames, IA 50011
dc.description.affiliationUnespDept. of Genetics IBB-UNESP, Botucatu/SP 18607-741
dc.format.extent3743-3751
dc.identifierhttp://dx.doi.org/10.1074/jbc.M510778200
dc.identifier.citationJournal of Biological Chemistry, v. 281, n. 6, p. 3743-3751, 2006.
dc.identifier.doi10.1074/jbc.M510778200
dc.identifier.issn0021-9258
dc.identifier.issn1083-351X
dc.identifier.scopus2-s2.0-33645649913
dc.identifier.urihttp://hdl.handle.net/11449/68779
dc.language.isoeng
dc.relation.ispartofJournal of Biological Chemistry
dc.relation.ispartofjcr4.010
dc.relation.ispartofsjr2,672
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectBiochemistry
dc.subjectEnzymes
dc.subjectGenes
dc.subjectMolecular biology
dc.subjectMolecular weight
dc.subjectMutagenesis
dc.subjectRNA
dc.subjectYeast
dc.subjectExons
dc.subjectIntrons
dc.subjectMitochondrial protein
dc.subjectMutants
dc.subjectProteins
dc.subjectcyclooxygenase 1
dc.subjectcyclooxygenase 24 enzyme
dc.subjectmitochondrial DNA
dc.subjectmitochondrial protein
dc.subjectunclassified drug
dc.subjectCOX24 protein, S cerevisiae
dc.subjectcytochrome c oxidase
dc.subjectepitope
dc.subjectfungal DNA
dc.subjecthemagglutinin
dc.subjectmessenger RNA
dc.subjectSaccharomyces cerevisiae protein
dc.subjectcontrolled study
dc.subjectenzyme localization
dc.subjectintron
dc.subjectmolecular cloning
dc.subjectnonhuman
dc.subjectNorthern blotting
dc.subjectopen reading frame
dc.subjectphenotype
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectprotein function
dc.subjectprotein processing
dc.subjectRNA splicing
dc.subjectSaccharomyces
dc.subjectsequence alignment
dc.subjectyeast
dc.subjectamino acid sequence
dc.subjectbiosynthesis
dc.subjectchemistry
dc.subjectenzymology
dc.subjectfungal gene
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectmitochondrion
dc.subjectmolecular genetics
dc.subjectmutation
dc.subjectSaccharomyces cerevisiae
dc.subjectsequence homology
dc.subjectspecies difference
dc.subjectAmino Acid Sequence
dc.subjectBlotting, Northern
dc.subjectCloning, Molecular
dc.subjectDNA, Fungal
dc.subjectDNA, Mitochondrial
dc.subjectElectron Transport Complex IV
dc.subjectEpitopes
dc.subjectGenes, Fungal
dc.subjectHemagglutinins
dc.subjectMitochondria
dc.subjectMitochondrial Proteins
dc.subjectMolecular Sequence Data
dc.subjectMutation
dc.subjectOpen Reading Frames
dc.subjectPhenotype
dc.subjectRNA, Messenger
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectSequence Homology, Amino Acid
dc.subjectSpecies Specificity
dc.titleCOX24 codes for a mitochondrial protein required for processing of the COX1 transcripten
dc.typeArtigo
dcterms.licensehttp://www.jbc.org/site/misc/Copyright_Permission.xhtml
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

Arquivos

Coleções

Artigos - Genética - IBB