A standartized research protocol for platelet-rich plasma (PRP) preparation in rats

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dc.contributor.authorMessora, Michel Reis
dc.contributor.authorNagata, Maria Jose Hitomi [UNESP]
dc.contributor.authorFurlaneto, Flávia Aparecida Chaves
dc.contributor.authorDornelles, Rita de Cassia Martins [UNESP]
dc.contributor.authorBonfim, Sueli Regina Mogami [UNESP]
dc.contributor.authorDeliberador, Tatiane Miranda
dc.contributor.authorGarcia, Valdir Gouveia [UNESP]
dc.contributor.authorBosco, Alvaro Francisco [UNESP]
dc.contributor.institutionCentro Universitário de Lavras
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Ceará (UFC)
dc.contributor.institutionUniversidade Positivo
dc.date.accessioned2016-01-28T16:54:03Z
dc.date.available2016-01-28T16:54:03Z
dc.date.issued2011
dc.description.abstractIntroduction: The urgent need for studies using standardized protocols to evaluate the real biological effects of PRP has been emphasized by several authors. Objective: The purpose of this study was to standardize a methodology for autologous Platelet-Rich Plasma (PRP) preparation in rats. Material and methods: Twentyfour, 5 to 6-month-old, male rats, weighing 450 to 500 g were used. After general anesthesia, 3.15 ml of blood was collected from each animal, via cannulation of the jugular vein. A standardized technique of double centrifugation was used to prepare PRP. PRP samples and peripheral blood platelets were then manually counted using a Neubauer chamber. Student’s t-test was used to compare the differences between the number of platelets in peripheral blood and PRP samples (p < 0.05). In addition, PRP and peripheral blood smears were stained to see platelets’ morphology. Results: All surgical procedures were well tolerated by the animals and they were healthy during the entire experimental period. PRP samples showed higher significantly platelet concentrations than peripheral blood samples (2,677,583 and 683,680 respectively). Conclusion: Within the limits of this study, it can be concluded that the method used produced autologous PRP with appropriated platelet quantity and quality, in rats.en
dc.description.affiliationSchool of Dentistry, University Center of Lavras – Lavras – Minas Gerais – Brazil
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento Cirurgia e Clínica Integrada, Faculdade de Odontologia de Araçatuba, Aracatuba, Rua José Bonifário, 1193, Vila Mendonça, CEP 16015-050, SP, Brasil
dc.description.affiliationSchool of Dentistry, Federal University of Ceara, Campus Sobral – Sobral – Ceara – Brazil
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento Ciências Básicas, Faculdade de Odontologia de Araçatuba, Aracatuba, Rua José Bonifário, 1193, Vila Mendonça, CEP 16015-050, SP, Brasil
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento Clínica Cirurgia e Reprodução Animal, Faculdade de Medicina Veterinária (FMVA), Aracatuba, Rua José Bonifário, 1193, Vila Mendonça, CEP 16015-050, SP, Brasil
dc.description.affiliationCourse and Program of Post-Graduation in Dentistry, Positivo University – Curitiba – Parana – Brazil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento Cirurgia e Clínica Integrada, Faculdade de Odontologia de Araçatuba, Aracatuba, Rua José Bonifário, 1193, Vila Mendonça, CEP 16015-050, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento Ciências Básicas, Faculdade de Odontologia de Araçatuba, Aracatuba, Rua José Bonifário, 1193, Vila Mendonça, CEP 16015-050, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento Clínica Cirurgia e Reprodução Animal, Faculdade de Medicina Veterinária (FMVA), Aracatuba, Rua José Bonifário, 1193, Vila Mendonça, CEP 16015-050, SP, Brasil
dc.format.extent305-310
dc.identifierhttp://www.univille.edu.br/pt-BR/departamentos/odontologia/revista-rsbo/edicoes--2011/RSBO---v8---n03/725748
dc.identifier.citationRSBO. Revista Sul-Brasileira de Odontologia, v. 8, n. 3, p. 305-310, 2011.
dc.identifier.issn1806-7727
dc.identifier.lattes4068921369233125
dc.identifier.lattes8233055882071695
dc.identifier.lattes4688659299176448
dc.identifier.lattes8399870097572073
dc.identifier.lattes5435902422784889
dc.identifier.lattes9831236034935598
dc.identifier.orcid0000-0003-0783-6612
dc.identifier.urihttp://hdl.handle.net/11449/133192
dc.language.isoeng
dc.relation.ispartofRSBO. Revista Sul-Brasileira de Odontologia
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectPlatelet countsen
dc.subjectGrowth factorsen
dc.subjectAnimal modelsen
dc.titleA standartized research protocol for platelet-rich plasma (PRP) preparation in ratsen
dc.typeArtigo
unesp.author.lattes4068921369233125
unesp.author.lattes8233055882071695
unesp.author.lattes4688659299176448
unesp.author.lattes8399870097572073
unesp.author.lattes9831236034935598
unesp.author.lattes5435902422784889[4]
unesp.author.orcid0000-0003-0783-6612[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt
unesp.departmentCirurgia e Clínica Integradapt
unesp.departmentCiências Básicaspt
unesp.departmentClínica Cirurgia e Reprodução Animalpt

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Artigos - Cirurgia e Clínica Integrada - FOA
Artigos - Ciências Básicas - FOA
Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA