Diversity of cry genes and genetic characterization of Bacillus thuringiensis isolated from Brazil

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dc.contributor.authorVilas-Bôas, Gislayne Trindade [UNESP]
dc.contributor.authorLemos, Manoel Victor Franco [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:07Z
dc.date.available2014-05-27T11:21:07Z
dc.date.issued2004-08-01
dc.description.abstractTwo hundred and eighteen Bacillus thuringiensis isolates from Brazil were characterized by the presence of crystal protein genes by PCR with primers specific to different cry and cyt genes. Among these isolates, 95 were selected according to their geographic origin for genetic characterization with the 16S rRNA gene, RAPD, and plasmid profile. Isolates containing cryl genes were the most abundant (48%) followed by the cry11 and cyt (7%) and cry8 genes (2%). Finally, 40.3% of the isolates did not produce any PCR product. The plasmid profile and RAPD analysis showed a remarkable diversity among the isolates of B. thuringiensis not observed in the 16S rRNA gene. These results suggest that the genetic diversity of B. thuringiensis species results from the influence of different ecological factors and spatial separation between strains generated by the conquest of different habitats.en
dc.description.affiliationDepto. de Biol. Apl. a Agropecuaria FCAV/UNESP, Via Acesso Prof. Paulo D. Castellane, Jaboticabal/SP, 14884-900
dc.description.affiliationDepartamento de Biologia Geral CCB UEL, CP 6001, Londrina/PR, 86051-990
dc.description.affiliationUnespDepto. de Biol. Apl. a Agropecuaria FCAV/UNESP, Via Acesso Prof. Paulo D. Castellane, Jaboticabal/SP, 14884-900
dc.format.extent605-613
dc.identifierhttp://dx.doi.org/10.1139/w04-052
dc.identifier.citationCanadian Journal of Microbiology, v. 50, n. 8, p. 605-613, 2004.
dc.identifier.doi10.1139/w04-052
dc.identifier.issn0008-4166
dc.identifier.lattes7179273060624761
dc.identifier.scopus2-s2.0-8644273112
dc.identifier.urihttp://hdl.handle.net/11449/67819
dc.identifier.wosWOS:000224734600007
dc.language.isoeng
dc.relation.ispartofCanadian Journal of Microbiology
dc.relation.ispartofjcr1.243
dc.relation.ispartofsjr0,579
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subject16S rRNA gene
dc.subjectBacillus thuringiensis
dc.subjectCry genes
dc.subjectEcology
dc.subjectGenetic diversity
dc.subjectPlasmid profile
dc.subjectRAPD
dc.subjectBiodiversity
dc.subjectCrystal structure
dc.subjectGenes
dc.subjectProteins
dc.subjectRNA
dc.subjectGenetic characterization
dc.subjectSpatial separation
dc.subjectBacteria
dc.subjectribosome RNA
dc.subjectRNA 16S
dc.subjectbacterium
dc.subjectgenetic variation
dc.subjectplasmid
dc.subjectpolymerase chain reaction
dc.subjectbacterial gene
dc.subjectbacterium isolate
dc.subjectBrazil
dc.subjectcry gene
dc.subjectcyt gene
dc.subjectgenetic variability
dc.subjectgeographic distribution
dc.subjecthabitat structure
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectrandom amplified polymorphic DNA
dc.subjectRNA gene
dc.subjectBacterial Proteins
dc.subjectBacterial Toxins
dc.subjectDNA, Ribosomal
dc.subjectEndotoxins
dc.subjectHemolysin Proteins
dc.subjectMolecular Sequence Data
dc.subjectPlasmids
dc.subjectPolymerase Chain Reaction
dc.subjectRandom Amplified Polymorphic DNA Technique
dc.subjectRNA, Ribosomal, 16S
dc.subjectSequence Analysis, DNA
dc.subjectVariation (Genetics)
dc.subjectSouth America
dc.subjectWestern Hemisphere
dc.subjectWorld
dc.subjectBacteria (microorganisms)
dc.subjectPosibacteria
dc.titleDiversity of cry genes and genetic characterization of Bacillus thuringiensis isolated from Brazilen
dc.typeArtigo
dcterms.licensehttp://www.nrcresearchpress.com/page/authors/information/rights
unesp.author.lattes7179273060624761
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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