Cytogenetic Mapping of rRNAs and Histone H3 Genes in 14 Species of Dichotomius ( Coleoptera, Scarabaeidae, Scarabaeinae) Beetles

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dc.contributor.authorCabral-de-Mello, Diogo Cavalcanti [UNESP]
dc.contributor.authorMoura, R. C.
dc.contributor.authorMartins, C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.date.accessioned2014-05-20T13:51:56Z
dc.date.available2014-05-20T13:51:56Z
dc.date.issued2011-01-01
dc.description.abstractStandard cytogenetic analyses and chromosomal mapping of the genes for 18S and 5S rRNAs and histone H3 were performed in 14 species of beetles of the genus Dichotomius (Coleoptera, Scarabaeidae, Scarabaeinae). Conserved karyotypes with 2n = 18 and biarmed chromosomes were observed in all species. Moreover, the presence of a large metacentric pair (pair 1) was characteristic in the studied species, evidencing a remarkable synapomorphy for this genus, which probably originated by an ancient fusion of 2 auto-somes while the ancestral sex-chromosome pair remained conserved. FISH showed that the 5S rRNA and histone H3 genes are located in the proximal region of pair 2, with the 2 genes co-located. However, the major rDNA cluster probed by the 18S rRNA gene mapped to 1-3 bivalents, being exclusively autosomal, associated with sex elements, or both. In most species, the major rDNA cluster was observed in pair 3, and it was frequently (64.3%) located in the distal region regardless of the chromosome. The conserved number and position of the 5S rDNA/H3 histone cluster seems to be an ancient pattern shared by all of the studied species. In contrast, the major rDNA clusters apparently tolerate distinct patterns of diversification in the karyotypes of the species that could be associated with small inversions, ectopic recombination, and transposition. Moreover, we reinforced the association/co-localization between the 5S rRNA and histone H3 genes in this group contributing thus to the knowledge about the chromosomal organization and diversification patterns of multigene families in beetles and insects. Copyright (C) 2011 S. Karger AG, Baselen
dc.description.affiliationUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Morfol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniversidade Federal de Pernambuco (UFPE), UPE, Dept Biol, Inst Ciencias Biol, Recife, PE, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Morfol, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent127-135
dc.identifierhttp://dx.doi.org/10.1159/000326803
dc.identifier.citationCytogenetic and Genome Research. Basel: Karger, v. 134, n. 2, p. 127-135, 2011.
dc.identifier.doi10.1159/000326803
dc.identifier.issn1424-8581
dc.identifier.lattes8858800699425352
dc.identifier.orcid0000-0003-3534-974X
dc.identifier.urihttp://hdl.handle.net/11449/18553
dc.identifier.wosWOS:000290305100008
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofCytogenetic and Genome Research
dc.relation.ispartofjcr1.587
dc.relation.ispartofsjr0,748
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectChromosomal evolutionen
dc.subjectFluorescence in situ hybridizationen
dc.subjectMultigene familyen
dc.subjectScarabaeidaeen
dc.titleCytogenetic Mapping of rRNAs and Histone H3 Genes in 14 Species of Dichotomius ( Coleoptera, Scarabaeidae, Scarabaeinae) Beetlesen
dc.typeArtigo
dcterms.licensehttp://www.karger.com/Services/RightsPermissions
dcterms.rightsHolderKarger
unesp.author.lattes8858800699425352[3]
unesp.author.orcid0000-0003-3534-974X[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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