The plant energy-dissipating mitochondrial systems: Depicting the genomic structure and the expression profiles of the gene families of uncoupling protein and alternative oxidase in monocots and dicots

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dc.contributor.authorBorecký, Jiří
dc.contributor.authorNogueira, Fábio T. S.
dc.contributor.authorDe Oliveira, Kívia A. P.
dc.contributor.authorMaia, Ivan de Godoy [UNESP]
dc.contributor.authorVercesi, Aníbal E.
dc.contributor.authorArruda, Paulo
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:49Z
dc.date.available2014-05-27T11:21:49Z
dc.date.issued2006-03-01
dc.description.abstractThe simultaneous existence of alternative oxidases and uncoupling proteins in plants has raised the question as to why plants need two energy-dissipating systems with apparently similar physiological functions. A probably complete plant uncoupling protein gene family is described and the expression profiles of this family compared with the multigene family of alternative oxidases in Arabidopsis thaliana and sugarcane (Saccharum sp.) employed as dicot and monocot models, respectively. In total, six uncoupling protein genes, AtPUMP1-6, were recognized within the Arabidopsis genome and five (SsPUMP1-5) in a sugarcane EST database. The recombinant AtPUMP5 protein displayed similar biochemical properties as AtPUMP1. Sugarcane possessed four Arabidopsis AOx1-type orthologues (SsAOx1a-1d); no sugarcane orthologue corresponding to Arabidopsis AOx2-type genes was identified. Phylogenetic and expression analyses suggested that AtAOx1d does not belong to the AOx1-type family but forms a new (AOx3-type) family. Tissue-enriched expression profiling revealed that uncoupling protein genes were expressed more ubiquitously than the alternative oxidase genes. Distinct expression patterns among gene family members were observed between monocots and dicots and during chilling stress. These findings suggest that the members of each energy-dissipating system are subject to different cell or tissue/organ transcriptional regulation. As a result, plants may respond more flexibly to adverse biotic and abiotic conditions, in which oxidative stress is involved. © The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.en
dc.description.affiliationCentro de Biologia Molecular e Engenharia Genética Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP
dc.description.affiliationDepartamento de Patologia Clínica (NMCE) FCM Universidade Estadual de Campinas (UNICAMP), 13083-970, Campinas, SP
dc.description.affiliationDepartamento de Genética e Evolução Instituto de Biologia Universidade Estadual de Campinas (UNICAMP), 13083-970, Campinas, SP
dc.description.affiliationInstituto de Biociências Departamento de Genética Universidade Estadual Paulista (UNESP), 18618-000, Botucatu, SP
dc.description.affiliationUnespInstituto de Biociências Departamento de Genética Universidade Estadual Paulista (UNESP), 18618-000, Botucatu, SP
dc.format.extent849-864
dc.identifierhttp://dx.doi.org/10.1093/jxb/erj070
dc.identifier.citationJournal of Experimental Botany, v. 57, n. 4, p. 849-864, 2006.
dc.identifier.doi10.1093/jxb/erj070
dc.identifier.issn0022-0957
dc.identifier.issn1460-2431
dc.identifier.lattes8649222099176162
dc.identifier.scopus2-s2.0-33644897887
dc.identifier.urihttp://hdl.handle.net/11449/68788
dc.language.isoeng
dc.relation.ispartofJournal of Experimental Botany
dc.relation.ispartofjcr5.354
dc.relation.ispartofsjr2,822
dc.relation.ispartofsjr2,822
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAlternative oxidase
dc.subjectExpression profile
dc.subjectOxidative stress
dc.subjectUncoupling proteins
dc.subjectGenes
dc.subjectPlants (botany)
dc.subjectProteins
dc.subjectSugar cane
dc.subjectTissue
dc.subjectDicots
dc.subjectMonocots
dc.subjectEnergy dissipation
dc.subjectalternative oxidase
dc.subjectcarrier protein
dc.subjection channel
dc.subjectmembrane protein
dc.subjectmessenger RNA
dc.subjectmitochondrial protein
dc.subjectmitochondrial uncoupling protein
dc.subjectoxidoreductase
dc.subjectvegetable protein
dc.subjectamino acid sequence
dc.subjectArabidopsis
dc.subjectbiology
dc.subjectcold
dc.subjectcomparative study
dc.subjectenzymology
dc.subjectgene expression profiling
dc.subjectgenetics
dc.subjectgenome
dc.subjectmetabolism
dc.subjectmitochondrion
dc.subjectmolecular genetics
dc.subjectmultigene family
dc.subjectphylogeny
dc.subjectphysiology
dc.subjectreverse transcription polymerase chain reaction
dc.subjectsequence alignment
dc.subjectsequence analysis
dc.subjectsugarcane
dc.subjectAmino Acid Sequence
dc.subjectCarrier Proteins
dc.subjectCold
dc.subjectComputational Biology
dc.subjectGene Expression Profiling
dc.subjectGenome, Plant
dc.subjectIon Channels
dc.subjectMembrane Proteins
dc.subjectMitochondria
dc.subjectMitochondrial Proteins
dc.subjectMolecular Sequence Data
dc.subjectMultigene Family
dc.subjectOxidoreductases
dc.subjectPhylogeny
dc.subjectPlant Proteins
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectSaccharum
dc.subjectSequence Alignment
dc.subjectSequence Analysis, Protein
dc.subjectEnergy
dc.subjectGenotypes
dc.subjectPlant Tissues
dc.subjectPlants
dc.subjectSugar Cane
dc.subjectArabidopsis thaliana
dc.subjectDicotyledoneae
dc.subjectLiliopsida
dc.subjectSaccharum hybrid cultivar
dc.subjectSaccharum sp.
dc.titleThe plant energy-dissipating mitochondrial systems: Depicting the genomic structure and the expression profiles of the gene families of uncoupling protein and alternative oxidase in monocots and dicotsen
dc.typeArtigo
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
unesp.author.lattes8649222099176162
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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