Simple and clean determination of tetracyclines by flow injection analysis

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dc.contributor.authorRodríguez, Michael Pérez [UNESP]
dc.contributor.authorPezza, Helena Redigolo [UNESP]
dc.contributor.authorPezza, Leonardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:37:32Z
dc.date.available2015-12-07T15:37:32Z
dc.date.issued2015-09-03
dc.description.abstractAn environmentally reliable analytical methodology was developed for direct quantification of tetracycline (TC) and oxytetracycline (OTC) using continuous flow injection analysis with spectrophotometric detection. The method is based on the diazo coupling reaction between the tetracyclines and diazotized sulfanilic acid in a basic medium, resulting in the formation of an intense orange azo compound that presents maximum absorption at 434nm. Experimental design was used to optimize the analytical conditions. The proposed technique was validated over the concentration range of 1 to 40μgmL(-1), and was successfully applied to samples of commercial veterinary pharmaceuticals. The detection (LOD) and quantification (LOQ) limits were 0.40 and 1.35μgmL(-1), respectively. The samples were also analyzed by an HPLC method, and the results showed agreement with the proposed technique. The new flow injection method can be immediately used for quality control purposes in the pharmaceutical industry, facilitating monitoring in real time during the production processes of tetracycline formulations for veterinary use.en
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Química Analítica, Instituto de Química de Araraquara
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent386-392
dc.identifierhttp://dx.doi.org/10.1016/j.saa.2015.08.048
dc.identifier.citationSpectrochimica Acta. Part A, Molecular And Biomolecular Spectroscopy, v. 153, p. 386-392, 2015.
dc.identifier.doi10.1016/j.saa.2015.08.048
dc.identifier.issn1873-3557
dc.identifier.lattes3192125098361121
dc.identifier.lattes5978908591853524
dc.identifier.orcid0000-0003-0197-7369
dc.identifier.pubmed26344484
dc.identifier.urihttp://hdl.handle.net/11449/131556
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofSpectrochimica Acta. Part A, Molecular And Biomolecular Spectroscopy
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectFlow injection analysis (fia)en
dc.subjectGreen chemistryen
dc.subjectQuantificationen
dc.subjectSpectrophotometryen
dc.subjectTetracyclinesen
dc.subjectVeterinary pharmaceuticalsen
dc.titleSimple and clean determination of tetracyclines by flow injection analysisen
dc.typeArtigo
dcterms.rightsHolderElsevier B. V.
unesp.author.lattes3192125098361121
unesp.author.lattes5978908591853524
unesp.author.orcid0000-0003-0197-7369[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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Artigos - Química Analítica - IQ