Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae)

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dc.contributor.authorMarcon, Helena Sanches [UNESP]
dc.contributor.authorCoscrato, Virgínia Elias [UNESP]
dc.contributor.authorSelivon, Denise
dc.contributor.authorPerondini, André Luiz Paranhos
dc.contributor.authorMarino, Celso Luis [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T15:07:44Z
dc.date.available2014-05-20T15:07:44Z
dc.date.issued2011-06-01
dc.description.abstractWolbachia are endosymbiont bacteria of the family Rickettsiacea that are widespread in invertebrates and occur between 20% and 60% of Neotropical insects. These bacteria are responsible for reproductive phenomena such as cytoplasmic incompatibility, male killing, feminization and parthenogenesis. Supergroups A and B of Wolbachia are common in insects and can be identified using primers for 16S rDNA, ftsZ and wsp; these primers vary in their ability to detect Wolbachia. The ftsZ primer was the first primer used to detect Wolbachia in Anastrepha fruit flies. The primers for 16S rDNA, ftsZ and wsp and the corresponding PCR conditions have been optimized to study the distribution of Wolbachia and their effect on the biology of Anastrepha in Brazil. In this work, we examined the ability of these primers to detect Wolbachia in Anastrepha populations from three regions in the State of São Paulo, southeastern Brazil. All of the samples were positive for Wolbachia supergroup A when screened with primers for 16S A rDNA and wsp A; the wsp B primer also gave a positive result, indicating cross-reactivity. The ftsZ primer showed a poor ability to detect Wolbachia in Anastrepha and generated false negatives in 44.9% of the samples. These findings indicate that reliable PCR detection of Wolbachia requires the use of primers for 16S rDNA and wsp to avoid cross-reactions and false negatives, and that the ftsZ primer needs to be redesigned to improve its selectivity.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Departamento de Genética
dc.description.affiliationUniversidade de São Paulo Instituto de Biociências Departamento de Genética e Biologia Evolutiva
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Departamento de Genética
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent778-785
dc.identifierhttp://dx.doi.org/10.1590/S1517-83822011000200046
dc.identifier.citationBrazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 2, p. 778-785, 2011.
dc.identifier.doi10.1590/S1517-83822011000200046
dc.identifier.fileS1517-83822011000200046.pdf
dc.identifier.issn1517-8382
dc.identifier.lattes0165348738208319
dc.identifier.orcid0000-0003-4524-954X
dc.identifier.scieloS1517-83822011000200046
dc.identifier.urihttp://hdl.handle.net/11449/26644
dc.identifier.wosWOS:000291614000046
dc.language.isoeng
dc.publisherSociedade Brasileira de Microbiologia
dc.relation.ispartofBrazilian Journal of Microbiology
dc.relation.ispartofjcr1.810
dc.relation.ispartofsjr0,630
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectAnastrephaen
dc.subjectcross reactionen
dc.subjectftsZen
dc.subjectwspen
dc.titleVariations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae)en
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bjm/paboutj.htm
unesp.author.lattes0165348738208319[5]
unesp.author.orcid0000-0003-4524-954X[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Genética - IBB