Leucine Supplementation Augments Insulin Secretion in Pancreatic Islets of Malnourished Mice

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dc.contributor.authorAmaral, Andressa G.
dc.contributor.authorRafacho, Alex [UNESP]
dc.contributor.authorde Oliveira, Camila A. Machado
dc.contributor.authorBatista, Thiago M.
dc.contributor.authorRibeiro, Rosane A.
dc.contributor.authorLatorraca, Marcia Q.
dc.contributor.authorBoschero, Antonio C.
dc.contributor.authorCarneiro, Everardo M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Mato Grosso (UFMT)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T15:33:12Z
dc.date.available2014-05-20T15:33:12Z
dc.date.issued2010-08-01
dc.description.abstractObjectives: We investigated the influence of leucine supplementation on insulin secretion and on some proteins related to insulin secretion in malnourished mice.Methods: Swiss mice (aged 21 days) received isocaloric normo-17% (NP) or 6% low-protein (LP) diet for 120 days. Half of the NP and LP mice received 1.5% leucine in the drinking water during the last 30 days (NPL and LPL, respectively).Results: The LP mice were hypoinsulinemic compared with the NP group, whereas LPL mice exhibited increased insulinemia in the fed state versus LP mice. The LP mouse islets were less responsive to 22.2 mM glucose, 100 KM carbachol (Cch), and 10 mM leucine than the NP group. However, LPL islets were more responsive to all these conditions compared with the LP group. The muscarinic type 3 receptor, (M3R) CaA2, and PKC-alpha protein contents were reduced in LP compared with NP islets but significantly higher in LPL than LP islets. The p-AKT/AKT ratio was higher in LPL compared with LP islets.Conclusions: Leucine supplementation increases insulin secretion in response to glucose and leucine and to agents that potentiate secretion, such as Cch, in malnourished mice. The enhanced levels of M3R, CaA2, and PKC-alpha proteins, as well as of the p-AKT/AKT ratio, may play a role in this process.en
dc.description.affiliationSão Paulo State Univ, Dept Phys Educ, Sch Sci, São Paulo, Brazil
dc.description.affiliationFed Univ Mato Grosso UFMT, Dept Food & Nutr, Sch Nutr, Cuiaba, Mato Grosso, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Anat Cell Biol & Physiol & Biophys, Inst Biol, Campinas, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Phys Educ, Sch Sci, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 07/50365-4
dc.description.sponsorshipIdFAPESP: 08/53811-8
dc.format.extent847-855
dc.identifierhttp://dx.doi.org/10.1097/MPA.0b013e3181d37210
dc.identifier.citationPancreas. Philadelphia: Lippincott Williams & Wilkins, v. 39, n. 6, p. 847-855, 2010.
dc.identifier.doi10.1097/MPA.0b013e3181d37210
dc.identifier.issn0885-3177
dc.identifier.urihttp://hdl.handle.net/11449/41904
dc.identifier.wosWOS:000280190900016
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofPancreas
dc.relation.ispartofjcr2.958
dc.relation.ispartofsjr1,327
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectinsulin secretionen
dc.subjectleucineen
dc.subjectlow-protein dieten
dc.subjectpancreatic isletsen
dc.subjectprotein levelsen
dc.titleLeucine Supplementation Augments Insulin Secretion in Pancreatic Islets of Malnourished Miceen
dc.typeArtigo
dcterms.licensehttp://edmgr.ovid.com/spine/accounts/copyrightTransfer.pdf
dcterms.rightsHolderLippincott Williams & Wilkins

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