Vitamin D Induces Increased Systolic Arterial Pressure via Vascular Reactivity and Mechanical Properties

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dc.contributor.authorSantos, Priscila Portugal dos [UNESP]
dc.contributor.authorMurino Rafacho, Bruna Paola [UNESP]
dc.contributor.authorGoncalves, Andrea de Freitas [UNESP]
dc.contributor.authorJaldin, Rodrigo Gibin [UNESP]
dc.contributor.authorNascimento, Thiago Bruder do
dc.contributor.authorBarbosa Silva, Marcondes Alves
dc.contributor.authorAssis Cau, Stefany Bruno
dc.contributor.authorRoscani, Meliza Goi [UNESP]
dc.contributor.authorGaiolla, Paula Schmidt Azevedo [UNESP]
dc.contributor.authorMinicucci, Marcos Ferreira [UNESP]
dc.contributor.authorTostes, Rita de Cassia
dc.contributor.authorZornoff, Leonardo Antonio Mamede [UNESP]
dc.contributor.authorPaiva, Sergio Alberto Rupp de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-12-03T13:10:30Z
dc.date.available2014-12-03T13:10:30Z
dc.date.issued2014-06-12
dc.description.abstractBackground/Aims: The aim of this study was to evaluate whether supplementation of high doses of cholecalciferol for two months in normotensive rats results in increased systolic arterial pressure and which are the mechanisms involved. Specifically, this study assesses the potential effect on cardiac output as well as the changes in aortic structure and functional properties.Methods: Male Wistar rats were divided into three groups: 1) Control group (C, n = 20), with no supplementation of vitamin D, 2) VD3 (n = 19), supplemented with 3,000 IU vitamin D/kg of chow; 3) VD10 (n = 21), supplemented with 10,000 IU vitamin D/kg of chow. After two months, echocardiographic analyses, measurements of systolic arterial pressure (SAP), vascular reactivity, reactive oxygen species (ROS) generation, mechanical properties, histological analysis and metalloproteinase-2 and -9 activity were performed.Results: SAP was higher in VD3 and VD10 than in C rats (p = 0.001). Echocardiographic variables were not different among groups. Responses to phenylephrine in endothelium-denuded aortas was higher in VD3 compared to the C group (p = 0.041). Vascular relaxation induced by acetylcholine (p = 0.023) and sodium nitroprusside (p = 0.005) was impaired in both supplemented groups compared to the C group and apocynin treatment reversed impaired vasodilation. Collagen volume fraction (<0.001) and MMP-2 activity (p = 0.025) was higher in VD10 group compared to the VD3 group. Elastin volume fraction was lower in VD10 than in C and yield point was lower in VD3 than in C.Conclusion: Our findings support the view that vitamin D supplementation increases arterial pressure in normotensive rats and this is associated with structural and functional vascular changes, modulated by NADPH oxidase, nitric oxide, and extracellular matrix components.en
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Internal Med, Botucatu Med Sch, Sao Paulo, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Surg & Orthopaed, Botucatu Med Sch, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Pharmacol, Sch Med Ribeirao Preto USP, Sao Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Dept Internal Med, Botucatu Med Sch, Sao Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Dept Surg & Orthopaed, Botucatu Med Sch, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 08/56489-0
dc.format.extent9
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0098895
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 9, n. 6, 9 p., 2014.
dc.identifier.doi10.1371/journal.pone.0098895
dc.identifier.fileWOS000338701300020.pdf
dc.identifier.issn1932-6203
dc.identifier.lattes5016839015394547
dc.identifier.lattes1213140801402647
dc.identifier.lattes7438704034471673
dc.identifier.orcid0000-0002-5843-6232
dc.identifier.urihttp://hdl.handle.net/11449/112195
dc.identifier.wosWOS:000338701300020
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPLOS ONE
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleVitamin D Induces Increased Systolic Arterial Pressure via Vascular Reactivity and Mechanical Propertiesen
dc.typeArtigo
dcterms.rightsHolderPublic Library Science
unesp.author.lattes1213140801402647[9]
unesp.author.lattes7438704034471673
unesp.author.orcid0000-0002-9446-0707[11]
unesp.author.orcid0000-0002-5980-4367[10]
unesp.author.orcid0000-0002-0442-739X[7]
unesp.author.orcid0000-0001-8825-6980[2]
unesp.author.orcid0000-0003-4412-1990[13]
unesp.author.orcid0000-0003-4419-2959[6]
unesp.author.orcid0000-0002-5843-6232[9]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Clínica Médica - FMB
Artigos - Cirurgia e Ortopedia - FMB