Single-cell expression profiling reveals a dynamic state of cardiac precursor cells in the early mouse embryo

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dc.contributor.authorKokkinopoulos, Ioannis
dc.contributor.authorIshida, Hidekazu
dc.contributor.authorSaba, Rie
dc.contributor.authorRuchaya, Prashant [UNESP]
dc.contributor.authorCabrera, Claudia
dc.contributor.authorStruebig, Monika
dc.contributor.authorBarnes, Michael
dc.contributor.authorTerry, Anna
dc.contributor.authorKaneko, Masahiro
dc.contributor.authorShintani, Yasunori
dc.contributor.authorCoppen, Steven
dc.contributor.authorShiratori, Hidetaka
dc.contributor.authorAmeen, Torath
dc.contributor.authorMein, Charles
dc.contributor.authorHamada, Hiroshi
dc.contributor.authorSuzuki, Ken
dc.contributor.authorYashiro, Kenta
dc.contributor.institutionQueen Mary University of London
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionOsaka University
dc.date.accessioned2015-12-07T15:32:27Z
dc.date.available2015-12-07T15:32:27Z
dc.date.issued2015
dc.description.abstractIn the early vertebrate embryo, cardiac progenitor/precursor cells (CPs) give rise to cardiac structures. Better understanding their biological character is critical to understand the heart development and to apply CPs for the clinical arena. However, our knowledge remains incomplete. With the use of single-cell expression profiling, we have now revealed rapid and dynamic changes in gene expression profiles of the embryonic CPs during the early phase after their segregation from the cardiac mesoderm. Progressively, the nascent mesodermal gene Mesp1 terminated, and Nkx2-5+/Tbx5+ population rapidly replaced the Tbx5low+ population as the expression of the cardiac genes Tbx5 and Nkx2-5 increased. At the Early Headfold stage, Tbx5-expressing CPs gradually showed a unique molecular signature with signs of cardiomyocyte differentiation. Lineage-tracing revealed a developmentally distinct characteristic of this population. They underwent progressive differentiation only towards the cardiomyocyte lineage corresponding to the first heart field rather than being maintained as a progenitor pool. More importantly, Tbx5 likely plays an important role in a transcriptional network to regulate the distinct character of the FHF via a positive feedback loop to activate the robust expression of Tbx5 in CPs. These data expands our knowledge on the behavior of CPs during the early phase of cardiac development, subsequently providing a platform for further study.en
dc.description.affiliationTranslational Medicine and Therapeutics, William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom
dc.description.affiliationTranslational Medicine and Therapeutics, William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom; Physiology and Pathology, University of São Paulo State - UNESP, Araraquara School of Dentistry, Araraquara, São Paulo, Brazil.
dc.description.affiliationClinical Pharmacology, William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom; Genome Centre, William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom; NIHR Barts Cardiovascular Biomedical Research Unit, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom
dc.description.affiliationGenome Centre, William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom.
dc.description.affiliationDepartment of Developmental Genetics, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
dc.description.affiliationUnespPhysiology and Pathology, University of São Paulo State - UNESP, Araraquara School of Dentistry, Araraquara, São Paulo, Brazil
dc.description.sponsorshipMedical Research Council (MRC)
dc.description.sponsorshipIdMRC: G0900105
dc.description.sponsorshipIdMRC: MR/J007625/1
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0140831
dc.identifier.citationPlos One, v. 10, n. 10, 2015.
dc.identifier.doi10.1371/journal.pone.0140831
dc.identifier.filePMC4607431.pdf
dc.identifier.issn1932-6203
dc.identifier.pmcPMC4607431
dc.identifier.pubmed26469858
dc.identifier.urihttp://hdl.handle.net/11449/131192
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourcePubMed
dc.titleSingle-cell expression profiling reveals a dynamic state of cardiac precursor cells in the early mouse embryoen
dc.typeArtigo
dcterms.rightsHolderPublic Library Science
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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