Color alteration, hydrogen peroxide diffusion, and cytotoxicity caused by in-office bleaching protocols

Página do item simplificado
dc.contributor.authorAlmeida, Letícia Cunha Amaral Gonzaga de [UNESP]
dc.contributor.authorSoares, Diana Gabriela de Souza [UNESP]
dc.contributor.authorGallinari, Marjorie Oliveira [UNESP]
dc.contributor.authorCosta, Carlos Alberto de Souza [UNESP]
dc.contributor.authorSantos, Paulo Henrique dos [UNESP]
dc.contributor.authorBriso, André Luiz Fraga [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-08-06T16:12:57Z
dc.date.available2015-08-06T16:12:57Z
dc.date.issued2014
dc.description.abstractObjectives This study evaluated the color alteration, cytotoxicity, and hydrogen peroxide (HP) diffusion associated with different in-office bleaching protocols. Materials and methods Bovine enamel/dentin disks were subjected to three bleaching sessions with 35 % HP (three 15-min applications), 35 % HP (one 45-min application), or 20 % HP (one 45-min application). The control group was not bleached. Before bleaching, the disks were adapted to artificial pulp chambers positioned in compartments containing 1 ml of acetate buffer or medium, so that the dentin remained in contact with these substances. Immediately after bleaching, the HP that diffused through the disks was stabilized by acetate buffer and was quantified (two-way repeated measures ANOVA/Fisher’s protected least significant difference (PLSD) test; α=5 %). Cells of mouse dental papilla cell-23 (MDPC-23) were incubated in this culture media for 1 h, followed by analysis of cellular metabolism (methyl tetrazolium assay) (one-way ANOVA/Tukey test; α=5 %) and morphology (scanning electron microscopy). The specimen color alteration (ΔE) was analyzed by reflection spectrophotometry (two-way repeated measures ANOVA/Fisher’s PLSD test; α=5 %). Results All protocols showed equal effectiveness at the end of the treatment. HP diffusion was significantly higher in the groups bleached with 35 % HP. Reapplication of 35 % HP resulted in increased diffusion only in the first session; however, the decrease in cell metabolism was similar for all studied protocols. Conclusion Despite greater peroxide diffusion in the groups treated with 35 % HP, all protocols showed the same effectiveness and were cytotoxic to MDPC-23 cells. Clinical relevance Bleaching protocols using high HP concentrations should be avoided because they exert aggressive actions on odontoblast-like cells.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Fisiologia e Patologia, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801903, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Fisiologia e Patologia, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801903, SP, Brasil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.description.sponsorshipIdFAPESP: 2010/10378-2
dc.description.sponsorshipIdFAPESP: 2010/17637-3
dc.description.sponsorshipIdFUNDUNESP: 0113610
dc.format.extent673–680
dc.identifierhttp://link.springer.com/article/10.1007%2Fs00784-014-1285-3
dc.identifier.citationClinical Oral Investigations, v. 19, n. 3, p. 673–680, 2014.
dc.identifier.doi10.1007/s00784-014-1285-3
dc.identifier.issn1436-3771
dc.identifier.lattes4517484241515548
dc.identifier.lattes0493479801083622
dc.identifier.orcid0000-0002-4100-5153
dc.identifier.urihttp://hdl.handle.net/11449/125726
dc.language.isoeng
dc.relation.ispartofClinical Oral Investigations
dc.relation.ispartofsjr0,986
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectCytotoxicityen
dc.subjectHydrogen peroxideen
dc.subjectBleaching agenten
dc.subjectCell cultureen
dc.titleColor alteration, hydrogen peroxide diffusion, and cytotoxicity caused by in-office bleaching protocolsen
dc.typeArtigo
unesp.author.lattes4517484241515548[4]
unesp.author.lattes0493479801083622[5]
unesp.author.lattes5761956467234702[6]
unesp.author.orcid0000-0002-4100-5153[5]
unesp.author.orcid0000-0002-6126-1760[6]
unesp.author.orcid0000-0002-7455-6867[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt
unesp.departmentOdontologia Restauradorapt
unesp.departmentMateriais Odontológicos e Prótesept
unesp.departmentFisiologia e Patologiapt

Arquivos

Coleções

Artigos - Fisiologia e Patologia - FOAR